We narrowed to 6,761 results for: SIM;

1. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

   Plasmid

   #208385

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgcGAS (Cgas Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKO2-CDK4

   Plasmid

   #215065

   Purpose

   Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Retroviral

   Tags

   mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-P3-Alb-Rosmarinus

   Plasmid

   #183462

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   Rosmarinus

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-P3-Alb-mCarmine

   Plasmid

   #183464

   Purpose

   To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mCarmine

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. iMb-Control-Mosaic (IR98.10)

   Plasmid

   #99748

   Purpose

   Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   MbYFP, MbTomato, MbKate2
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tre-Tight ifgMosaic Donor vector (LH121)

   Plasmid

   #99630

   Purpose

   To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   N-PhiM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   (Please see depositor comments below)

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hAR-R617M,K618M,K632M,K633M

   Plasmid

   #115778

   Purpose

   Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   Androgen Receptor (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R617M, K618M ,K632M, K633M, insertion of Q81 and …

   Promoter

   CMV

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroX GFP Mps1 DK

   Plasmid

   #63703

   Purpose

   Inducible expression of GFP-Mps1 DK fusion in mammalian cells

   Depositor

   Floris Foijer

   Article

   Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   Mps1 (TTK Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deletion of aa 47-156, kinetochore binding domain

   Promoter

   tight TRE promoter

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL2-hOct4 DE-SV40-Luc

    Plasmid

    #26279

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments below

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBKWH-Lam

    Plasmid

    #133899

    Purpose

    Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LMNA (Lam) (LMNA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

    Plasmid

    #114613

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 23 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOEM1_pCMV:bmp4-pPH:vsvged

    Plasmid

    #111157

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

    Insert

    hBmp4 (BMP4 Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-M4-AR-W741C-S650A

    Plasmid

    #171252

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-M4-AR-W741C-S650D

    Plasmid

    #171253

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

    Promoter

    CMV

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-crRNA(TARDBP)

    Plasmid

    #109323

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TARDBP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

    Plasmid

    #114626

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

    Plasmid

    #114611

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 23 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

    Plasmid

    #114617

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 23 polyQ repeat

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTWIN1-His6-Ssp-Httex1-43Q-A2C

    Plasmid

    #114622

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge