Addgene

Showing: 2831 - 2840 of 14547 results

Results per page:

Sort results by:

pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Article

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

6His, TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b/Shigella flexneri IpaA

Plasmid

#46178

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

IpaA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Use

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29a-human histone 4-in-One

Plasmid

#223237

Purpose

Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

Depositor

Weixing Zhao

Inserts

His6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII

Use

Tags

Dual StrepII, His6, TEV protease site, and VSVG

Expression

Bacterial

Mutation

A103G

Promoter

T7

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b_H6-Smt3-AesC-GFP

Plasmid

#234486

Purpose

Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

C-terminal Fragment of the Aes123 PolB1 Split Intein

Use

Tags

Hexahistidine Tag, Superfolder Green Fluorescent …

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-AviTag-DogCatcher-MBP

Plasmid

#171928

Purpose

Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogCatcher-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

DogCatcher added to N-terminus of MBP via a GSGES…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Use

Tags

6xHis-SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Use

Tags

6xHis tag and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b-UbcH5b/2419

Plasmid

#11475

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5b (UBE2D2 Human)

Use

Tags

Expression

Bacterial

Mutation

n/a

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only