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pGFP-AUC-IRES-mCherry

Plasmid

#222117

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AUC start codon

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5423_p3WJdB-glcO60

Plasmid

#221194

Purpose

DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Insert

T7 promoter-glcO60-3WJdB

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter with downstream glcO60 operator

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PLCd1(PHD)

Plasmid

#221753

Purpose

Stable expression of GFP-PLCd1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLCd1 (PLCD1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–175

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-2xFYVE

Plasmid

#221750

Purpose

Stable expression of GFP-2xFYVE in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

tandem HRS FYVE domain

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-YF

Plasmid

#210723

Purpose

Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pQE60 G121A wgMDH

Plasmid

#204252

Purpose

Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

G121A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196E wgMDH

Plasmid

#204272

Purpose

Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123A wgMDH

Plasmid

#204254

Purpose

Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

P123A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130Q wgMDH

Plasmid

#204268

Purpose

Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130Q

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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