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PiggyBac_8mer_pegArray_bb_pos.1

Plasmid

#238542

Purpose

Golden gate acceptor backbone for position 1 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.2

Plasmid

#238543

Purpose

Golden gate acceptor backbone for position 2 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.3

Plasmid

#238544

Purpose

Golden gate acceptor backbone for position 3 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pB-Orthogonal_LTR5Hs_CARGO

Plasmid

#248159

Purpose

Encodes a set of guide RNAs targeting LTR5Hs elements (orthogonal to Addgene #191316)

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhSTOP_EcoRI_EYFP

Plasmid

#250547

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with two editible stop codons

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhSTOP_EcoRI_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhSTOP_EcoRI_EYFP

Plasmid

#250548

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with one editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhSTOP_EcoRI_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhancedSTOP_1xBoxB_EYFP

Plasmid

#250545

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with two editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhancedSTOP_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_drePum1_HA_dreAdar1

Plasmid

#250541

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of zebrafish Pum1 fused to the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

drePum1_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_EYFP_3xBoxB_Recorder_SV40

Plasmid

#250534

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a recorder containing 3 box-b hairpins

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

EYFP_3xBoxB_Recorder_SV40

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhancedSTOP_1xBoxB_EYFP

Plasmid

#250543

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with one editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhancedSTOP_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhancedEDITED_1xBoxB_EYFP

Plasmid

#250544

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without an editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhancedEDITED_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1_E488Q

Plasmid

#250533

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zerbafish Adar1 (E488Q)

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1_E488Q

Use

Synthetic Biology

Mutation

E488Q

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1

Plasmid

#250532

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain on zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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CBE-Cas12a-GFP

Plasmid

#246533

Purpose

Plant co-editing vector

Depositor

Nian Wang

Article

Huang et al Nat Plants. 2023 Oct;9(10):1591-1597. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGR721 - SLD3p-TP-DNAP1.Badboy2

Plasmid

#222013

Purpose

Expresses the Badboy2 TP-DNAP1 variant under control of a weak promoter for reducing p1 copy number

Depositor

Chang Liu

Article

Rix et al Science. 2024 Nov 8;386(6722):eadm9073.

Insert

TP-DNAP1 BadBoy2

Expression

Yeast

Promoter

SLD3p

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGY240

Plasmid

#239811

Purpose

Express H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

H3
H2B

Use

Fungal expression

Tags

EGFP and mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY251

Plasmid

#239813

Purpose

Express H2B-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY203

Plasmid

#239802

Purpose

Express GUS in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

GUS

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY209

Plasmid

#239804

Purpose

Express mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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