We narrowed to 22,452 results for: Tes
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Plasmid#234345PurposeLibrary-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpKDepositorInsertType II dCas9 sgRNA
UseCRISPRExpressionBacterialPromoterlacUV5Available SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPH021
Plasmid#234346PurposeLibrary-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpKDepositorInsertsType II dCas9 sgRNA
Type II dCas9 sgRNA
UseCRISPRExpressionBacterialPromoterlacUV5Available SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
p3A3-Flag
Plasmid#234805PurposeMammalian expression of bovine arrestin-3 with C-terminal Flag tagDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2_Rluc-Y0x-Fluc
Plasmid#233614PurposeExpression of Rluc-Fluc reporter gene containing no YDepositorInsertRluc-Y0x-Fluc
UseLuciferaseAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2C
Plasmid#236535PurposeFor expression of NM2C (dog) in mammalian cellsDepositorInsertMYH14
TagsHAExpressionMammalianMutationWTAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2B-Flag
Plasmid#236534PurposeFor expression of NM2B (dog) - with HA and FLAG tags in mammalian cellsDepositorInsertMYH10
TagsFLAG and HAExpressionMammalianMutationWTAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO1645
Plasmid#235719PurposeProtein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteriaDepositorInsertp40phox PX domain (NCF4 Human)
TagsGSTExpressionBacterialMutationCys at 2, C85S, aa 2-149Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP
Plasmid#236134PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
TagsEGFPExpressionMammalianPromoterE1b minimal promoter with four ZNF35/ZNF250 bindi…Available SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW408 (ZNF35tar-compact)x8-H2B-GFP
Plasmid#236129PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
TagsEGFPExpressionMammalianPromoterE1b minimal promoter with eight ZNF35 binding sit…Available SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW369 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236145PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW407 (ZNF35/ZNF35-6bp spacers)x4-H2B-GFP
Plasmid#236137PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
TagsEGFPExpressionMammalianPromoterE1b minimal promoter with four ZNF35/ZNF250 bindi…Available SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW490 CMV-TO-UTRN-14ZF-realdeImmLink-NZF (FLP-IN)
Plasmid#236149PurposePlasmid encoding the UTRN-14 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-14ZF-deImmLink-NZF
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW392 CMV-TO-NZF-(GGS)x8-FRB(T2098L) (FLP-IN)
Plasmid#236157PurposeExpresses NZF transcriptional activation domain attached by a GS linker to the mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sitesDepositorInsertNZF-FRB
ExpressionMammalianMutationT2098L in FRBPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW387 (ZNF35/IKZF1tar-6bp spacers)x4-H2B-GFP
Plasmid#236138PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNFIKZF1 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
TagsEGFPExpressionMammalianPromoterE1b minimal promoter with four ZNF35/ZNFIKZF1 bin…Available SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7_AvrSr62-5
Plasmid#233532PurposeExpresses AvrSr62-5 in yeastDepositorInsertAvrSr62-5
TagsGAL4 BD, Myc tagExpressionYeastPromoterADH1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7_AvrSr62-1
Plasmid#233531PurposeExpresses AvrSr62-1 in yeastDepositorInsertAvrSr62-1
TagsGAL4 BD, Myc tagExpressionYeastPromoterADH1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N73_eMagBF_V5
Plasmid#235642PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5ExpressionMammalianMutationresidues 1-73Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_Fbl_N73_eMagBF_V5
Plasmid#235643PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.DepositorInsertALIBi N-terminal fragment
TagsFbl-N73- and eMagBF-V5ExpressionMammalianMutationresidues 1-73Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_downstream_sgRNA
Plasmid#235610PurposeEncodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus downstream sgRNA
UseCRISPRAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_upstream_sgRNA
Plasmid#235609PurposeEncodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus upstream sgRNA
UseCRISPRAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N73_eMagBF_V5_NES
Plasmid#235614PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5-NESExpressionMammalianMutationresidues 1-73Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_BirA
Plasmid#235634PurposeEncodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experimentsDepositorInsertBirA
TagsNES-V5ExpressionMammalianAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N65_eMagBF_V5_NES
Plasmid#235612PurposeEncodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5-NESExpressionMammalianMutationresidues 1-65Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N77_eMagBF_V5_NES
Plasmid#235616PurposeEncodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5-NESExpressionMammalianMutationresidues 1-77Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N99_eMagBF_V5_NES
Plasmid#235618PurposeEncodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
TagseMagBF-V5-NESExpressionMammalianMutationresidues 1-99Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7_Sr62NLRa
Plasmid#233528PurposeExpresses Sr62NLRa in yeastDepositorInsertSr62NLRa
TagsGAL4 AD, HA tagExpressionYeastPromoterADH1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WTSLFD
Plasmid#232980PurposeGalactose iduced expression of Gcn4 WTSLFD+ in yeastDepositorInsertGcn4 WTSLFD+
Tags3xHA and TEV cleavage siteExpressionYeastMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+HA
Plasmid#232990PurposeGalactose iduced expression of Gcn4 Aro3+HA in yeastDepositorInsertGcn4 Aro3+
Tags3xHA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+HA
Plasmid#232989PurposeGalactose iduced expression of Gcn4 Aro2+HA in yeastDepositorInsertGcn4 Aro2+
Tags3xHA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+GFP
Plasmid#233001PurposeGalactose iduced expression of Gcn4 Aro2+GFP in yeastDepositorInsertGcn4 Aro2+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only