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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2197_pPV2-ZF10-NS3-p65

Plasmid

#239700

Purpose

BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR3-SV40-puro

Plasmid

#240537

Purpose

Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR3

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR2-SV40-puro

Plasmid

#240536

Purpose

Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-UbC-mStayGold

Plasmid

#241378

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with UbC promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

UbC

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Bactin-mStayGold

Plasmid

#241380

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

Bactin

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-RSV-mStayGold

Plasmid

#241381

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with RSV promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

RSV

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CMV-mStayGold

Plasmid

#241383

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CMV promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-hEF1a-mStayGold

Plasmid

#241388

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

hEF1a

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-UbC-mStayGold

Plasmid

#241389

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with UbC promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

UbC

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CBh-mStayGold

Plasmid

#241390

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CBh

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-Bactin-mStayGold

Plasmid

#241391

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

Bactin

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-PGK-mStayGold

Plasmid

#241392

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CAG Donor

Plasmid

#241374

Purpose

Bxb1-GA donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CAG Donor

Plasmid

#241373

Purpose

Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-hEF1a-mStayGold

Plasmid

#241377

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

hEF1a

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-ShortCAG-mStayGold

Plasmid

#241385

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with ShortCAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

ShortCAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-PGK-mStayGold

Plasmid

#241384

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_UbC

Plasmid

#241375

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-SV40-mStayGold

Plasmid

#241382

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with SV40 promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_C-myc (4a)

Plasmid

#238907

Purpose

Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_C-myc

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-TurboID

Plasmid

#237885

Purpose

AAV vector for Cre-dependent expression of TurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

TurboID

Use

AAV and Cre/Lox

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239746

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-Tad-CBE(CCG3)

Plasmid

#235083

Purpose

Expresses SpRY-Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCA2)

Plasmid

#235069

Purpose

Expresses Tad-CBE(CCA2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCA2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCA1)

Plasmid

#235075

Purpose

Expresses Tad-CBE(GCA1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCA1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCT1)

Plasmid

#235076

Purpose

Expresses Tad-CBE(GCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCT2)

Plasmid

#235077

Purpose

Expresses Tad-CBE(GCT2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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