We narrowed to 18,599 results for: tec

1. pAHW/Joseph2-Multitagged Plasmid

   Plasmid

   #226725

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pAHW/Joseph2
   

   Use

   Drosophila s2 cell expression

   Tags

   HA, Myc, GFP, Flag, V5, 6xHis

   Expression

   Insect

   Mutation

   Promoter

   Act5C

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM_ΔndhD2.km

   Plasmid

   #185532

   Purpose

   a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

   Depositor

   Paula Tamagnini

   Article

   Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

   Insert

   nptII gene flanked by ndhD2 upstream and downstream regions
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEM_Δflv3.sm

   Plasmid

   #185533

   Purpose

   a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

   Depositor

   Paula Tamagnini

   Article

   Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

   Insert

   aadA gene flanked by flv3 upstream and downstream regions
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Use

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEGFP-C1-BBS8

   Plasmid

   #218716

   Purpose

   Expresses N-terminally EGFP-tagged BBS8 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS8 (TTC8 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ca-LiPPI-CLAPon1.0 (TEVp)

   Plasmid

   #213409

   Purpose

   Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   CaM-V5-TEVp
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Talin-T48-L432G-Cterminal-mEmerald

   Plasmid

   #202373

   Purpose

   Talin(DeltaR2-10)-L432G-mEmerald

   Depositor

   Pakorn Kanchanawong

   Article

   Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

   Insert

   Talin(DeltaR2-10)-L432G-mEmerald
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Talin-T58-L432G-Cterminal-mEmerald

   Plasmid

   #202372

   Purpose

   Talin(DeltaR4-10)-L432G-mEmerald

   Depositor

   Pakorn Kanchanawong

   Article

   Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

   Insert

   Talin(DeltaR4-10)-L432G-mEmerald
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Talin-T100-L432G-Cterminal-mEmerald

   Plasmid

   #202371

   Purpose

   Talin(FullLength)-L432G-mEmerald

   Depositor

   Pakorn Kanchanawong

   Article

   Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

   Insert

   Talin(FullLength)-L432G-mEmerald
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Talin-T46-L432G-Cterminal-mEmerald

    Plasmid

    #202374

    Purpose

    Talin(DeltaR4-12)-L432G-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(DeltaR4-12)-L432G-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNCS-Clover4-LUMABS-HA

    Plasmid

    #183486

    Purpose

    To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-HA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IBAR-Cry2PHR-WH2

    Plasmid

    #176605

    Purpose

    Cry2PHR with N-terminal IBAR domain and C-terminal WH2 domain

    Depositor

    Robert Hughes

    Article

    Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

    Insert

    IBAR-Cry2PHR-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cry2PHR-IBAR-WH2

    Plasmid

    #176603

    Purpose

    Cry2PHR with C-terminal IBAR and WH2 domains

    Depositor

    Robert Hughes

    Article

    Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

    Insert

    Cry2PHR-mCherry-IBAR-WH2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SEPLuc-E2A-cyOFP1

    Plasmid

    #141084

    Purpose

    precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEPLuc-E2A-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGS-BacA-21222-FLP-3xFLAG

    Plasmid

    #109165

    Purpose

    Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

    Insert

    Flp
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCML 221

    Plasmid

    #98587

    Purpose

    pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    MS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVP91A-pcaHG

    Plasmid

    #113766

    Purpose

    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

    Depositor

    John Lipscomb

    Article

    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

    Inserts

    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    

    Use

    Tags

    Met-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPLICS Mt-ER Long P2A

    Plasmid

    #164107

    Purpose

    Detect the long Mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SPLICS Mt-ER Short P2A

    Plasmid

    #164108

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SPLICS Mt-ER- PM Long P2A

    Plasmid

    #164109

    Purpose

    Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM-Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge