We narrowed to 23,522 results for: Spr;

1. LentiGuide Blast

   Plasmid

   #199622

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330 Pten

   Plasmid

   #59909

   Purpose

   pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330 Ctnnb1.1

   Plasmid

   #59911

   Purpose

   pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PE2-His (IK1822)

   Plasmid

   #170103

   Purpose

   T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

   Depositor

   Keith Joung

   Article

   Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

   Insert

   bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
   

   Use

   CRISPR

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Bacteria codon-optimized nSpCas9 (H840A) & M-…

   Promoter

   T7

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

   Plasmid

   #140005

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171379

   Purpose

   Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized Cas13X.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid

    #187953

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187957

    Purpose

    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid

    #187952

    Purpose

    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-CasRx-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker, HA-tag, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBA900

    Plasmid

    #122237

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs2 at the 3' end of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas-MmdCas12m-CBE1

    Plasmid

    #192284

    Purpose

    MmdCas12m-CBE1 expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m-CDA-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid

    #139993

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGmCherry-gRNA

    Plasmid

    #87110

    Purpose

    gRNA cloning vector with CAGmCherry

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pROS17

    Plasmid

    #107931

    Purpose

    TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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