We narrowed to 59,355 results for: cloning

1. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Jin Wang, Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVC-Ds-MCS-Ds

   Plasmid

   #102416

   Purpose

   Empty vector with a multiple cloning site flanked by Ds integration elements

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCl-MCS_miniCMV_EGFP

   Plasmid

   #134984

   Purpose

   The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   minCMV

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRA110

   Plasmid

   #209343

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mStayGold fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-L5-Kir2.1-mCherry-L2

   Plasmid

   #32669

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   Kir2.1-mCherry (Kcnj2 Mouse)
   

   Use

   Gateway cloning vector

   Available Since

   March 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYSD063

   Plasmid

   #212916

   Purpose

   Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Myc-His(-)-HSulf-1

   Plasmid

   #13003

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 1 (SULF1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. LeGO-mOrange2

   Plasmid

   #85212

   Purpose

   Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   mOrange2
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2R3z-AtU3b-tRNA-ccdB-gRNA

    Plasmid

    #118390

    Purpose

    Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    AtU3b promoter
    

    Use

    CRISPR

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LeGO-S2

    Plasmid

    #85211

    Purpose

    Lentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    T-Sapphire
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGR

    Plasmid

    #46002

    Purpose

    Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEN276

    Plasmid

    #69150

    Purpose

    Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

    Depositor

    Pierre Germon

    Article

    Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

    Insert

    luxCDABE
    

    Use

    Luciferase

    Promoter

    frr

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-NP-B/BRISBANE/60/2008

    Plasmid

    #187921

    Purpose

    Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Tags

    UNTAGGED

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/Myc-His(-)-HSulf-2

    Plasmid

    #13004

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 (SULF2 Human)
    

    Tags

    6x His and Myc

    Expression

    Mammalian

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJRA109

    Plasmid

    #209342

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC3-yeGFP

    Plasmid

    #176181

    Purpose

    yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    yEGFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-minP:luc2

    Plasmid

    #138354

    Purpose

    Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Luciferase
    

    Expression

    Mammalian

    Promoter

    minimal promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pf hPGK mc2 IRES puro

    Plasmid

    #206237

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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