We narrowed to 5,937 results for: plasmid dna
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Plasmid#119303PurposeExpresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc connected to P9 coil via TEVs cleavable linker
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
1M-Qt-antiGFP
Plasmid#206951PurposeMammalian expression of 1M-Qt EMcapsulins targeting GFPDepositorInsertMmMT3, QtEnc, antiGFP-intrabody
ExpressionMammalianAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)‐NeoR
Plasmid#65528PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1ExpressionMammalianAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf35
Plasmid#12723PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf35 (J3R Vaccinia Virus)
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf200
Plasmid#12888PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf200
ExpressionMammalianAvailable SinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMaRSC
Plasmid#89189PurposeThe pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.DepositorInsertFlag-L274G-T2A-mCherry (Mars1 Mouse)
Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
nLuc_AP4_TEVs_P3mS
Plasmid#119299PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
UseLuciferaseTagsMyc tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
AP4mS_PPVs_P3_cLuc
Plasmid#119300PurposeExpresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
TrHKI-pGFPN3
Plasmid#21918DepositorInsertTruncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid (HK1 Human)
TagsGFPExpressionMammalianMutationThis is the truncated human HKI cDNA (lacking exo…Available SinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV/myc/ER-αT9ib
Plasmid#110768PurposeExpresses ER intrabody against mouse and human TLR9DepositorInsertintrabody against mouse and human TLR9
TagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
ExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 K137R
Plasmid#118353PurposeThis plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K298R
Plasmid#118362PurposeThis plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
nLuc_AP10_PPVs_P9mS
Plasmid#119302PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
ExpressionWormPromoterodr-3Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221A
Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T142Q
Plasmid#108109PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIC3_cpGR2(GGS5)-F59
Plasmid#246053PurposeExpression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9DepositorInsertAcrIIC3_cpGR2-F59
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only