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SP_B6

Plasmid

#228156

Purpose

Plasmid contains the secretion signal peptide sequence of the human CREG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_O75629_CREG1_HUMAN:0-31

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_C1

Plasmid

#228157

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast MEL6 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P41947_MEL6_YEASX:0-18

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_C2

Plasmid

#228158

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast (S. mikatae) MEL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q11129_MEL_SACMI:0-18

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_C4

Plasmid

#228160

Purpose

Plasmid contains the secretion signal peptide sequence of the human AFAM gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P43652_AFAM_HUMAN:0-21

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_C5

Plasmid

#228161

Purpose

Plasmid contains the secretion signal peptide sequence of the human STRCL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_A6NGW2_STRCL_HUMAN:0-25

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_C6

Plasmid

#228162

Purpose

Plasmid contains the secretion signal peptide sequence of the human NELL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q92832_NELL1_HUMAN:0-21

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_A1

Plasmid

#228145

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast MEL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P04824_MEL1_YEASX:0-18

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_A2

Plasmid

#228146

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q876J2_SPR1_SACU7:0-20

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_A3

Plasmid

#228147

Purpose

Plasmid contains the secretion signal peptide sequence of the human ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P38248_ECM33_YEAST:0-19

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_A4

Plasmid

#228148

Purpose

Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q6NUI6_CHADL_HUMAN:0-30

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_A5

Plasmid

#228149

Purpose

Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_O14793_GDF8_HUMAN:0-18

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-1E11

Plasmid

#239083

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

Transmembrane domain of CEA20

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Alanine 457 to Phenylalanine, and deleted…

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-1E01

Plasmid

#239013

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of GBGT1

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Leucine 6 to phenylalanine, changed Leuci…

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_020_psbA_5'Hom_rev_Cr

Plasmid

#235945

Purpose

5'Homology psbA reverse in the M2 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

psbA 5'Homology reverse

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph0

Plasmid

#231897

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph1

Plasmid

#231898

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph2

Plasmid

#231899

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPZ1A9

Plasmid

#223835

Purpose

Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP>mNeonGreen>

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13A7

Plasmid

#223837

Purpose

Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13B7

Plasmid

#223838

Purpose

Expressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>StayGold>

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPmTagBFP2FrFAST

Plasmid

#223839

Purpose

Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP2>FrFAST

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_023_psbD_5'Hom_rev_Cr

Plasmid

#235948

Purpose

5'Homology psbD reverse in the M2 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

psbD 5'Homology reverse

Use

Synthetic Biology

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_022_rbcL_5'Hom_rev_Cr

Plasmid

#235947

Purpose

5'Homology rbcL reverse in the M2 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

rbcL 5'Homology reverse

Use

Synthetic Biology

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_1_010_psbD_5'Hom_Cr

Plasmid

#235788

Purpose

5' Homology psbD in the M1 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the psbD locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_1_005_rbcL_5'Hom_Cr

Plasmid

#235786

Purpose

5' Homology rbcL in the M1 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the rbcL locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaq

Plasmid

#236750

Purpose

Express HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

Gnaq (Gnaq Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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IK216

Plasmid

#233652

Purpose

Cre BFP Reporter; loxP-STOP-loxP-BFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

loxP-STOP-loxP-BFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_006_psbH_5'Hom_rev_Cr

Plasmid

#235940

Purpose

5' Homology psbH reversee in the M2 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

psbH 5'Homology reverse

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_4_022_aac6-aph2-marker

Plasmid

#235898

Purpose

aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

aac6-aph2-marker

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge