We narrowed to 15,965 results for: grna

1. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

   Plasmid

   #115486

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

   Plasmid

   #128749

   Purpose

   Expresses control gRNA

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

   Plasmid

   #179117

   Purpose

   Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

   Depositor

   Baljit Khakh

   Article

   Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

   Insert

   Fermt2 sgRNA
   

   Use

   AAV

   Promoter

   U6

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

   Plasmid

   #86987

   Purpose

   For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Cas9
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

   Plasmid

   #158987

   Purpose

   Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP160
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-p53 gRNA-Cas9-T2A-mCherry

   Plasmid

   #164256

   Purpose

   Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sg p53
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

   Plasmid

   #181788

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194283

   Purpose

   EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM124: pAAV-EFS-CasRx-VEGFA presgRNA

    Plasmid

    #166872

    Purpose

    AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-VEGFA presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

    Plasmid

    #162337

    Purpose

    Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6; mU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid

    #180017

    Purpose

    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

    Plasmid

    #117406

    Purpose

    SpyCas9 sgRNA 2 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

    Plasmid

    #164040

    Purpose

    Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    sgTS5-sgTS6-sgTS7
    

    Use

    CRISPR

    Promoter

    mouse U6

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

    Plasmid

    #117411

    Purpose

    AsCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    AsCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

    Plasmid

    #209781

    Purpose

    Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    cTNT
    

    Use

    AAV

    Tags

    HA and OLLAS

    Promoter

    cTNT

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

    Plasmid

    #209782

    Purpose

    Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    sgRNA
    

    Use

    AAV

    Tags

    HA and OLLAS

    Promoter

    cTNT

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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