-
Plasmid#190091PurposeExpresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-NlaCascade-NLS-6xHis
Plasmid#180213PurposeExpressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.ColiDepositorInsertNlaCas5-Cas8-Cas7
UseTags6XHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-hDDI2-D252N_pET11a
Plasmid#169122Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationD252NPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorInsertAbeta(MC1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b-MBPx-MBPx-SpyTag003
Plasmid#216291PurposeExpresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.DepositorInsertMBPx-MBPx-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp8
Plasmid#154758PurposeExpression of SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-ELP[V-150]
Plasmid#67015PurposeExpresses ELP[V-150] in E. coliDepositorInsertELP[V-150]
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET LIC cloning vector (2A-T)
Plasmid#29665DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS TIR-2 sfGFP
Plasmid#154468PurposepET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only