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VN1313 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_eGFP-wcKanR

Plasmid

#235110

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Publication

Guyot et al Comput Struct Biotechnol J. 2026 May 25;

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1314 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_eGFP-wcKanR

Plasmid

#235111

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Publication

Guyot et al Comput Struct Biotechnol J. 2026 May 25;

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1315 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_eGFP-wcKanR

Plasmid

#235112

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Publication

Guyot et al Comput Struct Biotechnol J. 2026 May 25;

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-WT

Plasmid

#242749

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)

Plasmid

#239353

Purpose

Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter.

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA(mut 8356-8370)

Expression

Mammalian

Mutation

MALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccacc…

Available since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_RPP21 (pAVA3259)

Plasmid

#239327

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPP21

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPP21 (RPP21 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_BRF2 (pAVA3258)

Plasmid

#239326

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting BRF2

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting BRF2 (BRF2 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_RPPH1 (pAVA3586)

Plasmid

#239328

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPPH1 (RPPH1 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC6 (pP18(T)_B11-AVA4069)

Plasmid

#239304

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC6

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC6 (EXOSC6 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC7 (pP18(T)_C4-AVA4066)

Plasmid

#239305

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC7

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC7 (EXOSC7 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC9 (pP18(T)_A7-AVA4072)

Plasmid

#239307

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC9

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC9 (EXOSC9 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DIS3 (pP18(T)_A5-AVA4073)

Plasmid

#239308

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DIS3

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DIS3 (DIS3 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_RBM7 (pAVA3250)

Plasmid

#239322

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RBM7

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RBM7 (RBM7 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC10 (pAVA3318)

Plasmid

#239295

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC10

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC10 (EXOSC10 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC2 (pP18(T)_C3-AVA4067)

Plasmid

#239301

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC2

Depositor

Andrei Alexandrov

Publication

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC2 (EXOSC2 Human)

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Publication

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-IRX5

Plasmid

#170693

Purpose

doxycycline-inducible overexpression of IRX5

Depositor

Paul Burridge

Publication

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

IRX5 (IRX5 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-Bik-delMBR-pEGFP-C1

Plasmid

#177432

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.

Depositor

David Andrews

Insert

Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)

Tags

Venus

Expression

Mammalian

Mutation

Bik MBR removed (after position 136)

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-tBid-2E-pEGFP-C1

Plasmid

#166747

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

Depositor

David Andrews

Publication

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

tBid-2E (Bid Mouse)

Tags

Venus

Expression

Mammalian

Mutation

2 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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FSW CHIP delU-box-Myc

Plasmid

#246723

Purpose

Neuron-specific expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

deletion of U-box domain

Promoter

Synapsin

Available since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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