We narrowed to 18,759 results for: nls
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Plasmid#232096PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
PBE-NG
Plasmid#229045PurposeModified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
lvl0-G-Gal4-VPR
Plasmid#229187Purposelvl 0 5 geneDepositorInsertGal4-VPR
UseSynthetic BiologyAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_SYCP3_nterm
Plasmid#222524PurposeCas9/sgRNA plasmid for targeting SYCP3DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9, SYCP3 sgRNA (SYCP3 Human, Synthetic)
UseCRISPRAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald
Plasmid#225949PurposeLentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralExpressionMammalianMutationC100APromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald
Plasmid#225950PurposeLentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralExpressionMammalianMutationAdditional cysteine inserted after Cys-100PromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CALU-WPRE-UbC-Emerald
Plasmid#225951PurposeLentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CALU-WPRE-UbC-mCherry
Plasmid#225952PurposeLentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-LNPK-WPRE-UbC-Emerald
Plasmid#225953PurposeLentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-LNPK-WPRE-UbC-mCherry
Plasmid#225954PurposeLentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4-WPRE-UbC-Emerald
Plasmid#225943PurposeLentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eA3A-BE5-(NG)-PX458-EGFP
Plasmid#229687PurposeCRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.DepositorTypeEmpty backboneUseCRISPRTagsEGFPExpressionMammalianAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3
Plasmid#229538PurposepMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.DepositorInsertCas3
UseCRISPRExpressionBacterial and YeastMutationcodon optimized for S.cerevisiaeAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH941
Plasmid#230846PurposeEf1a-DivA-BE-T2A-GFP-wPREDepositorInsertDivA-BE
UseLentiviralExpressionMammalianAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH810
Plasmid#230849PurposeEf1a-DivA-BE-T2A-BlastR-wPREDepositorInsertDivA-BE
UseLentiviralExpressionMammalianAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459-CTNNA1(α-catenin CRISPR KO)
Plasmid#229708PurposeKnockout of a-catenin in mammalian cells using CRISPR-Cas9 technologyDepositorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 KI sgRNA
Plasmid#207611PurposesgRNA for HaloTag insertion into the endogenous TCAB1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCAAAGTCTTCATACTGCAG
TagsNoneExpressionMammalianPromoterCMV and U6Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LC3B sgRNA
Plasmid#207556PurposepX330 expressing Cas9 and a sgRNA targeting the LC3B locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCACTGAATACCAGCGCCTAG
ExpressionMammalianPromoterCMV and U6Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold
Plasmid#226921PurposeEncoding SauriABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSauriCas9
UseAAVPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold
Plasmid#226933PurposeEncoding Nme2ABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nNme2Cas9
UseAAVPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG144
Plasmid#227760PurposeExpresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal121Met mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG124
Plasmid#227758PurposeExpresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationThr83Ser mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR-zrif1_exon8
Plasmid#194434PurposeRight (RR) TALEN for mutating zebrafish rif1 geneDepositorAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD-zrif1_exon8
Plasmid#194435PurposeLeft (DD) TALEN for mutating zebrafish rif1 geneDepositorAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2-sgCBS-1
Plasmid#230081PurposeCrispr knock out human CBS geneDepositorAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2-sgCBS-2
Plasmid#230082PurposeCrispr knock out human CBS geneDepositorAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
AP1muA single guide RNA
Plasmid#230030Purposeguide RNA for AP1muA tagging at the C-terminus in pX459DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAP1M1 guide (AP1M1 Human)
UseCRISPRAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rab11 single guide RNA
Plasmid#229683Purposeguide RNA for Rab11 tagging at the N-terminus in pX459DepositorInsertRab11 guide (RAB11A Human)
UseCRISPRAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PKMC356_pTRE-ActivinA_IRES_BFP_WPRE
Plasmid#225355PurposeLentiviral vector for ActivinA and BFP expressionDepositorAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE
Plasmid#225349PurposeLentiviral vector for Wnt3a and BFP expressionDepositorAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
aTY171_pPGK1_iRFP_WPRE
Plasmid#225351PurposeLentiviral vector for iRFP expressionDepositorInsertiRFP670
UseLentiviralExpressionMammalianAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_NC_chr2
Plasmid#214683PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)DepositorInsertdgRNA_Chr2
UseCRISPR and LentiviralAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Puro_hs_NC_chr1
Plasmid#214688PurposeLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralMutationPuromycin resistance cassette has silent mutation…Available sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgAMPKa2-Cas9-GFP
Plasmid#208050PurposeLentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2DepositorAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
Plasmid#223210PurposeLentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP
Plasmid#223214PurposeLentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgAMPKa1-Cas9-GFP
Plasmid#208049PurposeLentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1DepositorAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC129: pAAV.CMV-CasRx mCh
Plasmid#203442PurposePlasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activityDepositorInsertRfxCas13d-T2A-mCherry
UseAAV and CRISPRExpressionMammalianPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC131: pAAV.CMV-CasRx-VEGFA sgRNA array
Plasmid#203444PurposePlasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAsDepositorInsertsU6-VEGFA sgRNA array
RfxCas13d
UseAAVTagsHA and NLSExpressionMammalianPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA
Plasmid#203451PurposePlasmid expressing active and codon optimised Cas13e with non-targeting gRNADepositorInsertsU6-non targeting (NT) sgRNA
Cas13e
UseAAVTagsHA and NLSExpressionMammalianMutationCodon optimised using GenScript toolPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only