We narrowed to 6,092 results for: plasmid dna
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Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash2
Plasmid#74130PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTPRA-MAC
Plasmid#139632PurposeMAC-tagged gene expressionDepositorAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
nLYN267-gs10-NLS-VPR
Plasmid#188265PurposePlasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnLYN267 (LYN Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
nERb446-gs10-NLS-VPR
Plasmid#188263PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnERb446 (ESR1 Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
nDHFR173-gs10-NLS-VPR
Plasmid#188262PurposePlasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnDHFR173 (DHFR Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
hMEF2A K403R
Plasmid#118355PurposeThis plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.DepositorAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop
Plasmid#240256PurposeAttenuated PROTEUS vector with Nb139-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.DepositorInsertnanobody Nb139
TagsNLS-VP64ExpressionMammalianMutationVP64 = 4*VP16[aa437-447]PromoterSFV subgenomic promoterAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
MEN2A-MAC
Plasmid#139634PurposeMAC-tagged gene expressionDepositorAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1_CR
Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
TagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-G641E
Plasmid#59366PurposePlasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertNAT10 NM_024662 (NAT10 Human)
TagsFLAGExpressionMammalianMutationG641E and silent mutations to render the cDNA res…PromoterCMV-tetAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Grhl3
Plasmid#172869PurposeMammalian expression vector with myc-tagged mouse Grhl3 coding sequenceDepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaMK2rep
Plasmid#239622PurposeCaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.DepositorInsertSpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
TagsSpot, myc, and sGFP2ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK208_2xBRD9BD_Y57A-IFP2.0C
Plasmid#233733PurposeExpresses BRD9 Bromodomain as tandem double domain Y57A mutant fused to C-terminal part of IFP2.0 as negative control for plasmid 233734DepositorInsertBRD9 (BRD9 Human)
TagsIFP2.0 C-terminal partExpressionMammalianMutationBromodomain cloned twice in tandem with Y57A muta…PromoterCMVAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS732A/S910A
Plasmid#221642PurposeThis retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2DepositorInsertPTK2 (S732A/S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK double mutation S732A/S910A (dominant negativ…Available SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKP712/713A
Plasmid#216544PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2DepositorInsertPTK2 (P712/713A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation P712/713AAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKR177/178A
Plasmid#216543PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2DepositorInsertPTK2 (R177/178A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation R177/178AAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Alpha
Plasmid#177961PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)DepositorInsertSARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
ExpressionMammalianMutationdel69, 70, del144, D614G, N501Y, A570D, P681H, T7…Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only