We narrowed to 9,638 results for: Ada;

1. pAAV-hSyn-GRAB_CCK1.0

   Plasmid

   #208673

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IRES-puro-3xFLAG-NBR1

   Plasmid

   #204546

   Purpose

   Expresses 3xFLAG-tagged NBR1 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC_hfnCas12a_NR1_NR2

   Plasmid

   #176252

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL5.0-Anillin shRNA

   Plasmid

   #187270

   Purpose

   Lentiviral expression of shRNA targeting Anillin

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   shRNA for Anillin (ANLN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB-hPER2-mScarlet-I-CD4-bla

   Plasmid

   #179449

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

   Plasmid

   #163572

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-EMTB-TurboID-V5

   Plasmid

   #190736

   Purpose

   Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Tags

   Myc, TurboID, and V5

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

   Plasmid

   #119723

   Purpose

   AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Thomas Oertner

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Inserts

   CaMPARI2
   CaMPARI2
   

   Use

   AAV

   Tags

   NES-his

   Expression

   Mammalian

   Mutation

   F391W, L398V

   Promoter

   hSyn1

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. W118-1_Flag-hIRAK1

   Plasmid

   #180405

   Purpose

   lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   IRAK1 (IRAK1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

    Plasmid

    #198394

    Purpose

    eRF1 (AAQ mutant) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    eRF1 (ETF1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    mutated GGQ motif (G183/184A)

    Promoter

    CMV

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Expression

    Mutation

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2R3a-Tq2CFP-OcsT

    Plasmid

    #71268

    Purpose

    Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Tq2CFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-TEV-mClover3

    Plasmid

    #105795

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcsII-pb4-flag

    Plasmid

    #86768

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 4 (PSMB4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD40-His/V5-c-MycS62A

    Plasmid

    #45599

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc S62A (Myc Mouse)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    S62A

    Promoter

    CMV

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pPB-cT3G-cERP2-TSC22D3

    Plasmid

    #192916

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1_MeCP2(R306C)

    Plasmid

    #110188

    Purpose

    Expression of mouse MeCP2 (R306C) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arg 306 Cys

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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