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AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA

Plasmid

#220731

Purpose

AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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AiP2017 - pAAV-AiE0774m-minBG-iCre(R297T)-BGHpA

Plasmid

#220723

Purpose

AiE0774m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA

Plasmid

#220717

Purpose

AiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EcWRS-4xWtR-ITR-GFP

Plasmid

#240280

Purpose

Express AAV2 transgene (EGFP) and EcWRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Publication

Pham et al Angew Chem Int Ed Engl. 2026 Jan 1:e1615

Inserts

AAV2-ITR-EGFP
EcWRS
EcWtR

Use

AAV

Expression

Mammalian

Available since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVss-CBA-H2B-staygold-SYNpA

Plasmid

#245085

Purpose

Fluorescent reporter gene

Depositor

Nicole Déglon

Publication

Rybarikova et al Gene Ther. 2025 Jul 28. doi: 10.1038/s41

Insert

H2B-StayGold

Use

AAV

Expression

Mammalian

Promoter

CBA

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713-P2A-GFP

Plasmid

#197172

Purpose

Expresses the protein of mRhubarb713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubard713-P2A-GFP

Use

AAV

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2-P2A-GFP

Plasmid

#197155

Purpose

Expresses the protein of miRFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2-P2A-GFP

Use

AAV

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP670-2-P2A-GFP

Plasmid

#197170

Purpose

Expresses the protein of miRFP670-2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-P2A-GFP

Use

AAV

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP670-P2A-GFP

Plasmid

#197169

Purpose

Expresses the protein of iRFP670-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP670-P2A-GFP

Use

AAV

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-E2-Crimson-P2A-GFP

Plasmid

#197162

Purpose

Expresses the protein of E2-Crimson-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

E2-Crimson-P2A-GFP

Use

AAV

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP703-P2A-GFP

Plasmid

#197158

Purpose

Expresses the protein of emiRFP703-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703-P2A-GFP

Use

AAV

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-IFP2-P2A-GFP

Plasmid

#197161

Purpose

Expresses the protein of IFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

IFP2-P2A-GFP

Use

AAV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb720-P2A-GFP

Plasmid

#197159

Purpose

Expresses the protein of mRhubarb720-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb720-P2A-GFP

Use

AAV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6-P2A-GFP

Plasmid

#197154

Purpose

Expresses the protein of BDFP1.6-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6-P2A-GFP

Use

AAV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb719-P2A-GFP

Plasmid

#197152

Purpose

Expresses the protein of mRhubarb719-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb719-P2A-GFP

Use

AAV

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-JNKKTR -miRFP670-WPRE

Plasmid

#159976

Purpose

single wavelength translocating reporter of the JNK pathway

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

JNKKTR -miRFP670

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AiP12701: pAAV-hChATp-SYFP2-WPRE3-BGHpA (Alias: CN2701)

Plasmid

#236601

Purpose

hChATp is a promoter fragment with activity in striatal cholinergic neurons

Depositor

Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14824: pAAV-AiE0441h_3xC2-minBG-iCre-BGHpA (Alias: CN4824)

Plasmid

#214861

Purpose

Expression of iCre in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

Plasmid

#210709

Purpose

This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Publication

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN (STAT1, MRTFA Human, Streptococcus pyogenes, Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Mutation

N55K in MCP

Promoter

U6

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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AiP1758 - pAAV-AiE2434m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220666

Purpose

AiE2434m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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