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pRExT2A-NT-PTy

Plasmid

#232202

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Publication

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::lasI

Plasmid

#189576

Purpose

Las synthase (3OC12-HSL)

Depositor

Daniel Ducat

Publication

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo5

Plasmid

#203956

Purpose

Cloning plasmid containing the chloramphenicol resistance gene insert

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Chloramphenicol Resistance Gene

Expression

Plant

Promoter

CAT promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo9

Plasmid

#203960

Purpose

Cloning plasmid containing the tetracycline resistance gene insert

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Tetracycline Resistance Gene

Expression

Plant

Promoter

CAT promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo12

Plasmid

#203982

Purpose

Cloning plasmid containing the rrnB T1 terminator insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

rrnB T1 Terminator

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK125

Plasmid

#200137

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Publication

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

OM-Tom70 (2-98)

Use

Synthetic Biology

Expression

Bacterial

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK106

Plasmid

#200132

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Publication

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Kar2-SS

Use

Synthetic Biology

Expression

Bacterial

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLBC0080

Plasmid

#172786

Purpose

Level 0 module containing NT2_nVenus

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

3X FLAG CtmVENUS 210-238 RSIAT pICH41258 (SP NT2)

Use

Synthetic Biology

Mutation

no

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0060

Plasmid

#172802

Purpose

Level 1 module containing 35S-nYFP-HSP21_P2 F

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-3XFLAGnYFP-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0070

Plasmid

#172803

Purpose

Level 1 module containing 35S-cYFP-HSP21_P3 F

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-cYFP3XHA-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0020

Plasmid

#172776

Purpose

Level 0 module containing NT1_OEP7

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS of chloroplast outer envelope membrane protein OEP7

Use

Synthetic Biology

Mutation

no

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo_TtoC and hairpin extension

Plasmid

#167925

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPD882 FKBP-ZF43 (NES)

Plasmid

#138854

Purpose

Constitutive expression of FKBP-ZF43 (NES) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43 (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD881 VPR-FRB (NES)

Plasmid

#138853

Purpose

Constitutive expression of VPR-FRB (NES) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD880 VP64-FRB (NES)

Plasmid

#138852

Purpose

Constitutive expression of VP64-FRB (NES) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-FRB (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD354 VP64-FRB (NLS)

Plasmid

#138846

Purpose

Constitutive expression of VP64-FRB under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPD353 VP16-FRB (NLS)

Plasmid

#138845

Purpose

Constitutive expression of VP16-FRB under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPD341 FKBP-ZF43 (NLS)

Plasmid

#138844

Purpose

Constitutive expression of FKBP-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPD171 ZF43(AARR)i

Plasmid

#138831

Purpose

Constitutive expression of ZF43(AARR)i under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43(AARR)i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A

Promoter

CMV

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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