We narrowed to 59,355 results for: cloning

1. pUPD-Cyc1t (17-21)

   Plasmid

   #101700

   Purpose

   Cyc1t in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT
   

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUPD-pTEF2 (01-11)

   Plasmid

   #101685

   Purpose

   pTEF2 in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT
   

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFH49

   Plasmid

   #128211

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBBR5pemIK-GW

   Plasmid

   #126556

   Purpose

   Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

   Depositor

   Lindsey Burbank, Drake Stenger

   Article

   Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pL0M-S-mClover3-EC18157

   Plasmid

   #137076

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFOSGE_COF4A02

   Plasmid

   #71021

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Ube3a (Ube3a Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

7. p663-UBC-miniTurbo-V5-STK3_IDG-K

   Plasmid

   #189879

   Purpose

   Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   STK3 (STK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p663-UBC-miniTurbo-V5-MARK3_IDG-K

   Plasmid

   #170583

   Purpose

   Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MARK3 (MARK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRL2960

   Plasmid

   #70694

   Purpose

   pDU1-E. coli shuttle vector (Km-r) with extended polylinker

   Depositor

   Peter Wolk

   Article

   Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

   Type

   Empty backbone

   Use

   Bacterial shuttle vector

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

    Plasmid

    #189903

    Purpose

    Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MAP3K14 (MAP3K14 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pF CAG mc2 IRES hygro

    Plasmid

    #206234

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMC-X2

    Plasmid

    #176152

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC-X3

    Plasmid

    #176153

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-XI1

    Plasmid

    #176155

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMC-XI5

    Plasmid

    #176158

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMC-XII2

    Plasmid

    #176160

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMINTC3GH (Apr)

    Plasmid

    #110078

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GGE020

    Plasmid

    #120777

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid

    #168207

    Purpose

    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Type

    Empty backbone

    Use

    Unspecified; Pdest gateway

    Tags

    V5 APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge