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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 37,795 results for: KAN

Showing: 5961 - 5980 of 37795 results

  3. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid
    #202531
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    DGKZ (DGKZ Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202531-plasmid-map-sequence-id-399624

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  4. t1-405

    Plasmid
    #166127
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    Tln1Head (1-405) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405 only
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166127-plasmid-map-sequence-id-330252

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  5. pGem-sHyper:sAPX

    Plasmid
    #84738
    Purpose
    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    pFMV/p35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84738-plasmid-map-sequence-id-180317

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  7. pGem-nHyPer:sAPX

    Plasmid
    #84735
    Purpose
    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    FMV/35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84735-plasmid-map-sequence-id-180316

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  17. DHFR-C-HiBiT

    Plasmid
    #207119
    Purpose
    Bacterially expressed DHFR-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)
    Use
    Tags
    10xHis-tag and HiBiT tag
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207119-plasmid-map-sequence-id-417165

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  18. pOET3-6xHis-hSMSr-ΔSAMD

    Plasmid
    #236225
    Purpose
    Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells
    Depositor
    Chiaki Murakami
    Article
    Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    Hexa histidine tag (6xHis-tag) and enterokinase s…
    Expression
    Insect
    Mutation
    sterile alpha motif domain at N-terminal (1-231 b…
    Promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236225-plasmid-map-sequence-id-471035

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  19. pCR4_miniFXN_7

    Plasmid
    #234360
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Marek Napierala
    Article
    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Expression
    Mutation
    There is a partial deletion in the 5'UTR of …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234360-plasmid-map-sequence-id-464761

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Showing: 5961 - 5980 of 37795 results