We narrowed to 5,134 results for: PID
-
Plasmid#213719PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GST-EGFP-GPIDAF
Plasmid#213716PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
UseTagsGSTExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPIDAF
Plasmid#213722PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIDAF
Plasmid#213708PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-sgRNA-mCherry-Puro sgPIDD
Plasmid#211528PurposeDeletes PIDDDepositorInsertsgPIDD
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP1-VP1-588TAG
Plasmid#240122PurposeExpress AAV2 capsid with ncAA mutant at site 588 of VP1DepositorInsertsRC2-DelVP1
VP1-588-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP
Plasmid#240124PurposeExpress AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporationDepositorInsertsAAV2-ITR-EGFP
MbPylRS
PylOtR
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-KO-delVP1-VP1-588TAG
Plasmid#240123PurposeExpress AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 588 of VP1DepositorInsertsRC2-DelVP1-R585,588A
VP1-588-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP12-VP12-454TAG
Plasmid#240061PurposeExpress AAV2 capsid with ncAA mutant at site 454 of VP1 and VP2DepositorInsertsRC2-DelVP12
VP1-454-TAG
VP2-454-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP2-VP2-454TAG
Plasmid#240060PurposeExpress AAV2 capsid with ncAA mutant at site 454 of VP2DepositorInsertsRC2-DelVP2
VP2-454-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP1-VP1-454TAG
Plasmid#240059PurposeExpress AAV2 capsid with ncAA mutant at site 454 of VP1DepositorInsertsRC2-DelVP1
VP1-454-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-KO-delVP1-VP1-454TAG
Plasmid#240062PurposeExpress AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 454 of VP1DepositorInsertsRC2-DelVP1-R585,588A
VP1-454-TAG-R585,588A
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG369
Plasmid#165606PurposeVector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)DepositorInsertω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
UseCRISPR and Synthetic BiologyTags1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…ExpressionBacterialMutationRecoding of BsaI site in the AmpR cassette, two B…PromoterlacUV2 driving ω-dCas9 and UV5 driving sgRNAAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS2696-CMV-Nme2SmuCas9-D56R
Plasmid#228717PurposeExpresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cellsDepositorInsertNme2SmuCas9-D56R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2697-CMV-Nme2SmuCas9-E932R
Plasmid#228718PurposeExpresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cellsDepositorInsertNme2SmuCas9-E932R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS-SAP30
Plasmid#109212PurposeEncodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression systemDepositorInsertHuman full-length SAP30 (SAP30 Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJuly 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
iSpyMac
Plasmid#155014PurposeExpresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cellsDepositorInsertiSpyMac
UseTagsExpressionMammalianMutationPID grafted onto SpyCas9 N-terminal domain, R221K…PromoterEF1ɑAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only