We narrowed to 72 results for: Toxoplasma gondii
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Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
TagsHAAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
Promotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLinker-2xTy-HXGPRT-LoxP
Plasmid#86664PurposeCRISPR tagging with 2xTy in Toxoplamsa gondiiDepositorInsertLinker-2xTy-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLinker-BirA-3xHA-HXGPRT-loxP
Plasmid#86668Purposefor generation of BirA tagged strains using CRISPR tagging technologyDepositorInsertLinker-BirA-3xHA-HXGPRT 3'UTR
ExpressionBacterialAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFloxed DHFR-TS*
Plasmid#100605PurposepUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTRDepositorInsertFloxed TgDHFR-TS*
UseToxoplasma expressionMutationPyrimethamine resistanceAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
TagsFLAG, HA, and V5ExpressionMammalianPromoterCMVAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-UPRT
Plasmid#69310Purposeexpresses UPRT cDNADepositorInsertUPRT cDNA
TagsHAExpressionBacterialAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPRT::Floxed DHFR-TS*
Plasmid#100606PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.DepositorInsertTgUPRT::Floxed TgDHFR-TS*
UseToxoplasma expressionMutationPyrimethamine resistancePromoterpTgDHFR-TSAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PP-ROP8
Plasmid#25123PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-ROP2A
Plasmid#25130PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert33.m01398:C43601
TagsHisExpressionBacterialAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CyP8
Plasmid#25122PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00136:C37419
TagsHisExpressionBacterialAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
A5TSR
Plasmid#50358Purposeexpresses T.gondii MIC2(67-593) domains in mammalian cellsDepositorInsertMIC2-A5TSR
TagsHISExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCHICK beta-actin promoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Scarlett-1_mAID_HXGPRT
Plasmid#259419PurposeAuxin-inducible degradation in T. gondiiDepositorInsertsScarlett-1
HXGPRT
UseUnspecifiedAvailable sinceAug. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
PP-mACP
Plasmid#25124PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert59.m03510:C40207
TagsHisExpressionBacterialAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tg-UBC (TgTwinScan_2218)
Plasmid#25565PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTg-UBC (TgTwinScan_2218)
TagsHisExpressionBacterialAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits