We narrowed to 786 results for: PFL

1. LLP453 pEf1a-NLS-aGCN4-mCherry

   Plasmid

   #100945

   Purpose

   mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, mCherry
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP109 pEF1a-gRNA-GFP

   Plasmid

   #100935

   Purpose

   GFP, puromycin and gRNA scaffold driven by EF1a promoter

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   gRNA scaffold
   

   Use

   Grna scaffold

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP251 pEF1a-UNC5C-4-SunTag

   Plasmid

   #100940

   Purpose

   TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE target sequence for UNC5C, SunTag array (10xGCN4)
   

   Use

   TALEN

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP338 pEF1a-DACH1-1-SunTag

   Plasmid

   #100942

   Purpose

   TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE target sequence for DACH1, SunTag array (10xGCN4)
   

   Use

   TALEN

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP457 pGK-dCas9-Suntag-BFP

   Plasmid

   #100957

   Purpose

   BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA and BFP

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP245 pEF1a-TALE-DNMT3a-Wt

   Plasmid

   #100937

   Purpose

   Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
   

   Use

   TALEN

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP185 pLVP-dCas9-DNMT3a V2

   Plasmid

   #100936

   Purpose

   dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

   Plasmid

   #100941

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
   

   Use

   Tags

   1xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

   Plasmid

   #100944

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   F636A, E660A, E725A, R295A

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP249 pGK-dCas9-DNMT3a V3

    Plasmid

    #100938

    Purpose

    dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge