We narrowed to 7,067 results for: ef1a;

1. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-mitoGFP-DIO

   Plasmid

   #174112

   Purpose

   Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

   Depositor

   Maarten Kole

   Article

   Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

   Insert

   mitoGFP (COX8A Human)
   

   Use

   AAV

   Tags

   Cox8 targeting sequence and GFP

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-IRES-Neo

   Plasmid

   #85139

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

   Plasmid

   #231986

   Purpose

   Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   OVA-2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF0432 Ef1a-Dn29-2a-EGFP

   Plasmid

   #193459

   Purpose

   Expression of Dn29 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-EF1aH Yap S127A

   Plasmid

   #86497

   Purpose

   Mammalian expression of FLAG-tagged Yap containing S127A mutation

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes associated protein 1 (YAP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-AAVS1-Ef1a-Puro

   Plasmid

   #227266

   Purpose

   Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO EYFP

   Plasmid

   #27056

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-4mtGCaMP6f-DIO

    Plasmid

    #179529

    Purpose

    Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Insert

    4mtGCaMP6f
    

    Use

    AAV

    Tags

    4x Cox8 targeting sequence

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAF0035 Ef1a-Nm60-2a-EGFP

    Plasmid

    #193464

    Purpose

    Expression of Nm60 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-Con/Fon oG

    Plasmid

    #131778

    Purpose

    INTRSECT 2.0

    Depositor

    Karl Deisseroth

    Article

    Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

    Insert

    rabies glycoprotein - oG
    

    Use

    AAV; Intrsect

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid

    #128317

    Purpose

    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Has Service

    AAV1

    Insert

    NES-JRGECO1a
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRE-VEGFA EF1a-rtTA3

    Plasmid

    #195881

    Purpose

    Lentiviral construct of doxycycline inducible VEGFA

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    VEGFA (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid

    #220800

    Purpose

    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

    Depositor

    Elizabeth Kirby

    Article

    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

    Insert

    Mars1 (Mars1 Mouse)
    

    Use

    Lentiviral

    Tags

    2xFLAG and T2A-mCherry

    Expression

    Mutation

    mutation in MetRS to change aa 274 from L to G

    Promoter

    EF1a

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOTTC293 - pAAV EF1a V5-synuclein (WT)

    Plasmid

    #60057

    Purpose

    An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    alpha-synuclein (SNCA human, Human)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Ef1a-fDIO mCherry

    Plasmid

    #114471

    Purpose

    Flp-dependent mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge