We narrowed to 2,854 results for: 623

1. pGP-CMV-jGCaMP8f

   Plasmid

   #162373

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Lyn-ICUE3

   Plasmid

   #61623

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   Lyn-ICUE3 (RAPGEF3 Human)
   

   Use

   Tags

   ECFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCKC014

   Plasmid

   #226623

   Purpose

   pLenti wt hTdT template for library 3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   Terminal deoxynucleotidyl transferase (DNTT Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

   Plasmid

   #69623

   Purpose

   A FRET sensor for Galphai1 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Inserts

   Gbeta-2A-cpV-Ggamma2
   Galphai-mTurquoise2
   

   Use

   Tags

   ciruclar permutated Venus (cp173V) and mTurquoise…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (IRES)

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

   Plasmid

   #86230

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-HA-CAD

   Plasmid

   #46238

   Purpose

   For expression of HA and FLAG-tagged murine CAD in mammalian cells

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Use

   Tags

   Strep Sumo

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4.TO-ORF73-2xCSTREP

   Plasmid

   #136232

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF73
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4.TO-ORF69-2xCSTREP

    Plasmid

    #136230

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF69

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF69
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GRB2-SHC

    Plasmid

    #139623

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBa.GFP-KIF13B tail 442-1843

    Plasmid

    #134623

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4.TO-ORF74-2xNSTREP

    Plasmid

    #136233

    Purpose

    Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF74

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF74
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA4.TO-ORF75-2xCSTREP

    Plasmid

    #136234

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF75

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF75
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA4.TO-ORF70-2xCSTREP

    Plasmid

    #136231

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF70

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF70
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB80-iLID-mCherry-RAB11

    Plasmid

    #174623

    Purpose

    Optogenetic coupling to recycling endosomes (RAB11) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB11 (RAB11A Synthetic, Human)
    

    Use

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB11: Met1Del

    Promoter

    Chicken beta-actin

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459_HR_Prnp_3

    Plasmid

    #78623

    Purpose

    pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    HR_Prnp_3 (Prnp Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3147

    Plasmid

    #144623

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB43 (ZBTB43 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-CS-154

    Plasmid

    #166230

    Purpose

    lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

    Depositor

    Nozomu Yachie

    Article

    Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

    Inserts

    U6-gRNA EGFP targetting
    nCas9-PmCDA1-UGI-2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV promoter and Human U6

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

    Plasmid

    #235608

    Purpose

    Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge