Addgene

We narrowed to 864 results for: F10;

Showing: 581 - 600 of 864 results

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PHF20 (3QII)

Plasmid

#32039

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PHF20 (PHF20 Human)

Use

Tags

His (with TEV cleavage)

Expression

Bacterial

Mutation

Tudor domain 2 only (amino acids 83-150)

Promoter

T7

Available since

Aug. 8, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pFLxTd5

Plasmid

#167519

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, tdKatushka2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-tdKatushka2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLuxB

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pXD80RFP-pXD68Kan2-RFP

Plasmid

#225329

Purpose

RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFVGe2

Plasmid

#149500

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, GmR, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-GmR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PVanCC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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CMYA5tnC/pDEST26-N-FLAG

Plasmid

#98130

Purpose

Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector

Depositor

Rita Shiang

Insert

CMYA5 (CMYA5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGL1_37

Plasmid

#199044

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP45-2A(sfGFP)

Plasmid

#126989

Purpose

Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSL3287

Plasmid

#139750

Purpose

Base Cas12a CRISPRi plasmid

Depositor

Himadri Pakrasi

Article

Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCBL101

Plasmid

#199722

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

NPTII

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF5

Plasmid

#17984

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF5 (TAF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pVimentin-PSmOrange

Plasmid

#31922

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Vimentin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pJUMP25-1A(sfGFP)

Plasmid

#126972

Purpose

Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p1153

Plasmid

#222930

Purpose

Shuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable marker

Depositor

Brian Wickes

Article

Determann B et al J Fungi (Basel). 2024 Jul 11;10(7):477.

Insert

CEN 4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPRi-D2

Plasmid

#182927

Purpose

inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

Depositor

Himadri Pakrasi

Article

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

ddcpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKeratin-PSmOrange

Plasmid

#31924

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Keratin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-Tubulin

Plasmid

#31919

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Tubulin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pMyosin-PSmOrange

Plasmid

#31921

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Myosin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pFRR5

Plasmid

#167506

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFRG5

Plasmid

#149486

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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