Addgene

Showing: 581 - 600 of 2236 results

Results per page:

Sort results by:

pMXs-IP HA-Parkin

Plasmid

#38248

Depositor

Noboru Mizushima

Article

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV DCC

Plasmid

#16459

Depositor

Bert Vogelstein

Article

Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

Insert

DCC (DCC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hyPE2

Plasmid

#177173

Purpose

Expresses hyPE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Song et al Nat Commun. 2021 Sep 23;12(1):5617. doi:

Insert

hyperPE2

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY1461_SERPINA1_CCA32_5avidity Cre

Plasmid

#193193

Purpose

Express Cre conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Promoter

Available since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_CO

Plasmid

#190335

Purpose

Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE2 with codon-optimized reverse transcriptase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

Plasmid

#24549

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP71-IFN-gamma

Plasmid

#174602

Purpose

Constitutive expression of secreted murine IFN gamma

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Constitutive expression of secreted murine IFN gamma

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al bioRxiv 2023.08.29.555275

Insert

qMaLioffG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Inducible Tac promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pMP71-tCD19-SIINFEKL

Plasmid

#174601

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, SIINFEKL (ovalbumin epitope)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GADD45 WT-luc

Plasmid

#8356

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter WT (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-efSaCas9-BlastR

Plasmid

#220971

Purpose

Expresses human codon-optimized efSaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

efSaCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL(CMV)EGFP/CMV/WPRE∆U3

Plasmid

#41790

Purpose

Lentiviral vector for CMV promoter driven expression of EGFP

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ACTIN-SOX2-PGK Cre

Plasmid

#59019

Purpose

Bicistronic Lentivirus expressing sox2 and Cre

Depositor

Trudy Oliver

Article

Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

B-actin

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

Plasmid

#188991

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Use

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Tornado-F30-Squash-Broccoli

Plasmid

#177918

Purpose

Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Squash-Broccoli

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170810

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge