We narrowed to 1,772 results for: rpe

1. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-CtIP

   Plasmid

   #109403

   Purpose

   Expresses Cas9 fusion to human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   full length CtIP

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

   Plasmid

   #218508

   Purpose

   integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mNeongreen
   

   Use

   Tags

   modified ssrA degradation tag

   Expression

   Bacterial

   Mutation

   deletion of MCS containing Plac_lacZa, introducti…

   Promoter

   P23

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC150-KEIMA-VAPA

   Plasmid

   #212096

   Purpose

   Fluorescent reporter for VAPA clearance to acidic lysosomes

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   VAPA (VAPA Human)
   

   Use

   Piggybac

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-HE

   Plasmid

   #109400

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-TAP(FLAG-HA) CYLD

   Plasmid

   #60031

   Purpose

   CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Retroviral

   Tags

   FLAG, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Use

   Tags

   Expression

   Mutation

   Geminin degron fragment

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-APPSw;T719N

   Plasmid

   #177094

   Purpose

   For lentiviral transduction of APPsw; T719N

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   APPsw;T719N (APP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T719N, K651N and M652L (please see depositor comm…

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR221-APP751

   Plasmid

   #193772

   Purpose

   Entry vector for human APP isoform having 751 amino acid residues

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   APP (APP Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

    Plasmid

    #145766

    Purpose

    Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.

    Depositor

    Marcel Bruchez

    Article

    Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021

    Insert

    kappa-myc-dK-2xG4S-TMst
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The dK FAP is dL5 with mutations at E50K, L89S, a…

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

    Plasmid

    #75082

    Purpose

    An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Nuc-eYFP
    

    Use

    AAV and Cre/Lox

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

    Plasmid

    #75081

    Purpose

    An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Mem-AcGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitylation site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLU_115

    Plasmid

    #225795

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Tn5]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGLU_79

    Plasmid

    #225780

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[abr]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGLU_97

    Plasmid

    #225810

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Himar]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKS1024

    Plasmid

    #239495

    Purpose

    Express peroxisomal RsAS

    Depositor

    John Dueber

    Insert

    Ubi-Y-RsAS-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIT6EG7m

    Plasmid

    #185885

    Purpose

    A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS1025

    Plasmid

    #239496

    Purpose

    Express inactive peroxisomal RsAS as negative control

    Depositor

    John Dueber

    Insert

    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H360E

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CTV

    Plasmid

    #131590

    Purpose

    Plasmid for the targeting of chloramphenicol gene

    Depositor

    Joon Tae Park

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p6108 pSG5-HPV31-E2

    Plasmid

    #24602

    Depositor

    Peter Howley

    Article

    Powell et al J Virol. 2010 Feb 24. ():.

    Insert

    HPV31 E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2010

    Availability

    Academic Institutions and Nonprofits only