165,652 results

1. pUAS-NanoLuc

   Plasmid

   #87696

   Purpose

   Gal4VP16 driven expression of NanoLuc

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NanoLuc
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_CRF1.0

   Plasmid

   #208655

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Lyn-AKAR4

   Plasmid

   #61620

   Purpose

   Lipid raft-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   Lyn-AKAR4
   

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-MBP-2xNLS-tdTomato

   Plasmid

   #104054

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-tdTomato
   

   Use

   AAV

   Tags

   NLS

   Promoter

   MBP

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. σ2-mCherry

   Plasmid

   #186579

   Purpose

   Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP2S1 (AP2S1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-OsTIR1(F74G)

   Plasmid

   #140730

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDae079 (eMutaT7[transition])

   Plasmid

   #187622

   Purpose

   Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Inserts

   TadA-8e-T7RNAP
   PmCDA1-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Flag FKHR

   Plasmid

   #13507

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   FKHR (FOXO1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-3XHA-EGFP-OMP25

    Plasmid

    #83356

    Purpose

    For tagging mitochondria with HA epitopes (HA-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XHA-EGFP-OMP25
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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