We narrowed to 4,188 results for: Pet2

1. 5VBT (UBC)

   Plasmid

   #101261

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   UBC (UBC Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MSP969

   Plasmid

   #98802

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYFHS-TEV T146S, D148P, S153N, S170A, N177M
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T146S, D148P, S153N, S170A, N177M

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK_proNR2_LGK.1

   Plasmid

   #81161

   Purpose

   Expresses levoglucosan kinase variant LGK.1 with promoter proNR2

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al ACS Synth Biol. 2015 Sep 14.

   Insert

   Levoglucosan kinase
   

   Use

   Tags

   His6x

   Expression

   Bacterial

   Mutation

   LGK.1 (L140I,S142A,A373C)

   Promoter

   proNR2

   Available since

   Sept. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK606

   Plasmid

   #72529

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK566

   Plasmid

   #71586

   Purpose

   Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) (YfdE)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK290

   Plasmid

   #71554

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes lysine-39 to alanine and tyrosine-260 to …

   Promoter

   T7

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK326

   Plasmid

   #71557

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-368 to aspartate

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK325

   Plasmid

   #71556

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-367 to aspartate

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK500

   Plasmid

   #72245

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and endogenous

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK345

    Plasmid

    #71559

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletes alanine-367 and alanine-368

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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