We narrowed to 12,704 results for: sequence

1. pOpen-TEV protease

   Plasmid

   #165509

   Purpose

   Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   TEV protease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDEST-PB-R4R3

   Plasmid

   #139518

   Purpose

   Provides two PiggyBac transposon sequences between gene cassettes in the destination vector

   Depositor

   Minoru Ko

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-Eco31I

   Plasmid

   #165506

   Purpose

   Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Eco31I
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. CMV-VGF

   Plasmid

   #107575

   Purpose

   Encodes the entire VGF cDNA sequence under the control of CMV promoter. Allows expression of VGF in mammalian cells.

   Depositor

   Andrea Levi

   Insert

   VGF (Vgf Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBACH2.2.0-gDNA

   Plasmid

   #176375

   Purpose

   Expresses spCas9 and gRNA sequence targeting BACH2

   Depositor

   Barbara Stranger, Kevin White

   Insert

   BACH2 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2 CBS4 (GB2718)

   Plasmid

   #160601

   Purpose

   Copper dependent CUP2 binding DNA sequence

   Depositor

   Diego Orzaez

   Insert

   CBS4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOT2-poly-cis

   Plasmid

   #141177

   Purpose

   To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

   Depositor

   Guiliang Tang

   Type

   Empty backbone

   Use

   RNAi and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKG333

   Plasmid

   #69751

   Purpose

   Retroviral expression of Histone H3

   Depositor

   Iain Cheeseman

   Insert

   Histone H3
   

   Use

   Retroviral

   Tags

   eGFP-TEV-S

   Expression

   Mammalian

   Mutation

   e.coli optimized sequence

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSOX2.1.0-gDNA

   Plasmid

   #176382

   Purpose

   Expresses spCas9 and gRNA sequence targeting SOX2

   Depositor

   Barbara Stranger, Kevin White

   Insert

   SOX2 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-EcoRIM

    Plasmid

    #165515

    Purpose

    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    M.EcoRI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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