We narrowed to 7,534 results for: ski

1. pCAG-2dV-Camui (wt)

   Plasmid

   #220366

   Purpose

   Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pERM2-nhpSer

   Plasmid

   #201922

   Purpose

   Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   SepRS(2)
   Sep-tRNA v2.0
   SerB
   EF-Sep
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   E412P, E414F, T417K, P495M, I496W, F529S and H67R…

   Promoter

   GlnS (constitutive), OXB20 (constitutive), lpp (c…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

   Plasmid

   #210709

   Purpose

   This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HA-GR

   Plasmid

   #215094

   Purpose

   Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   NR3C1 (NR3C1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY0216 PASTEv1 PE2-P2A-Bxb1

   Plasmid

   #179104

   Purpose

   PASTEv1 PE2-P2A-Bxb1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-PE2-P2A-Bxb1
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B Human, Synthetic, HDR template)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-HO1-2A-EGFP

   Plasmid

   #74672

   Purpose

   Mammalian expression of HO1 and EGFP

   Depositor

   Roberto Giovannoni

   Article

   Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

   Insert

   HO1 (HMOX1 Human)
   

   Tags

   2A-EGFP

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Cyclin D1

   Plasmid

   #215050

   Purpose

   Expresses EGFP-Cyclin D1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-CDT2

    Plasmid

    #215091

    Purpose

    Expresses FLAG-CDT2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDT2 (DTL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2 TDP-43 5F-L YFP

    Plasmid

    #84914

    Purpose

    Mammalian expresion of TDP-43 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    F147L, F149L, F194L, F229L, F231L

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Cyclin D3

    Plasmid

    #215052

    Purpose

    Expresses EGFP-Cyclin D3 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

    Plasmid

    #204821

    Purpose

    Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

    Depositor

    Emma Rawlins

    Article

    He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

    Insert

    An upstream region including human SCGB3A2 promoter
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SCGB3A2 promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-GFP-URA3-GFP

    Plasmid

    #72606

    Purpose

    Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

    Depositor

    Bruce Granger

    Article

    Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

    Inserts

    GFP (full)
    URA3
    GFP (partial)
    Optional Linker
    

    Mutation

    Includes full 819 bp coding sequence of URA3, 435…

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. A24M04 mIgM heavy chain-pFEEKW

    Plasmid

    #234809

    Purpose

    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

    Depositor

    Susan Pierce

    Article

    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    A24M04 membrane IgM heavy chain (IGHM Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EEK promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHLS-EF1a-FRB-SpCas9-A

    Plasmid

    #138477

    Purpose

    Expresses FRB fused SpCas9 for NanoMEDIC packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    SpCas9, human codon optimized
    

    Use

    CRISPR

    Tags

    3x HA tag and FRB

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. A24M04 Ig kappa light chain-pFEEKW

    Plasmid

    #234810

    Purpose

    Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

    Depositor

    Susan Pierce

    Article

    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    A24M04 Igkappa light chain (IGKC, IGK Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EEK promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe.puro.CDK8.flag

    Plasmid

    #19758

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. TurboID-Cyclin D1

    Plasmid

    #215084

    Purpose

    Expresses TurboID-Cyclin D1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    TurboID

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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