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pKG-GW1-RER2

Plasmid

#203170

Purpose

Yeast expression vector for yeast RER2

Depositor

William Sessa

Publication

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

RER2 (RER2 Budding Yeast)

Expression

Yeast

Promoter

TDH3

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGAD424-Rabex-5

Plasmid

#197043

Purpose

Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

Rabex-5 (RABGEF1 Bovine)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Publication

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Expression

Bacterial

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pC-HYG-YR

Plasmid

#61765

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Publication

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Hygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG303-Gal-GA50

Plasmid

#84907

Purpose

induce expression of dipeptide repeat GA in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GA50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Publication

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)

Plasmid

#14844

Depositor

Erin O'Shea

Publication

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-C2 (PHO5 Budding Yeast)

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTA…

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pYX233-b2-DHFR

Plasmid

#163759

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

Depositor

Johannes Herrmann

Publication

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2-DHFR (Dhfr Mouse, Budding Yeast)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-FKBP(M)x4

Plasmid

#85003

Purpose

Expresses EGFP-FKBP(M)x4 in yeast

Depositor

Benjamin Glick

Publication

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

EGFP-FKBP(M)x4

Expression

Yeast

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Publication

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pRS1244

Plasmid

#247644

Purpose

Inducible CRISPRi-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Publication

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1074

Plasmid

#247642

Purpose

Constitutive CRISPRi-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Publication

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1242

Plasmid

#247643

Purpose

Inducible CRISPRa-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Publication

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pMYT095

Plasmid

#198866

Purpose

CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Publication

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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