We narrowed to 16,261 results for: igh

1. mTagBFP2-Vimentin-7

   Plasmid

   #55333

   Purpose

   Localization: Intermediate Filaments, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. CROPseq-tRFP

   Plasmid

   #230938

   Purpose

   Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

   Depositor

   Hyejung Won

   Article

   Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

   Plasmid

   #110559

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   rhaSR-PrhaBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ220-Ultra

   Plasmid

   #176889

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   AsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET41a iGluu

   Plasmid

   #119834

   Purpose

   Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluu
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S72T

   Promoter

   T7

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pλPR cI-Elysis

   Plasmid

   #176891

   Purpose

   Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

   Depositor

   James Inglese

   Article

   Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

   Insert

   Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6c-V106W

   Plasmid

   #215826

   Purpose

   Express CBE6c V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209653

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB445

    Plasmid

    #74831

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLeAPS-GFP-blast

    Plasmid

    #182230

    Purpose

    Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    GFP-P2A-Blasticidin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB402

    Plasmid

    #74796

    Purpose

    Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid

    #115775

    Purpose

    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    

    Use

    Tags

    EGFP; dsReD

    Expression

    Mammalian

    Mutation

    gp140 uncleaved (1190-91aa, KR > TG; truncated…

    Promoter

    HIV-1 (LTR)

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CVS-N2c-mTurquoise

    Plasmid

    #172376

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    mTurquoise
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. B2094_CRY2olig-miRFP

    Plasmid

    #215114

    Purpose

    Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    CRY2olig-miRFP (CRY2 Mustard Weed)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB404

    Plasmid

    #74798

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sec61b-HA-mNG21-10

    Plasmid

    #141213

    Purpose

    Membrane Tethered Split Fluorescent Reporter for ER Retragrade

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    Sec61b-HA-mNG21-10
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGWB414

    Plasmid

    #74808

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TC1681

    Plasmid

    #164872

    Purpose

    CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OmpA-mNG-sfTq2ox

    Plasmid

    #124220

    Purpose

    Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    OmpA-mNG-sfTq2ox
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a_Pro-IL-1β

    Plasmid

    #214340

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1B (IL1B Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p16 nlsGPS2

    Plasmid

    #228093

    Purpose

    Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

    Depositor

    Alexander Jones

    Article

    Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

    Insert

    nlsGPS2
    

    Use

    Tags

    6xHis-T7 XpressTag and cMyc

    Expression

    Plant

    Mutation

    Promoter

    p16

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCerulean3-Rap1

    Plasmid

    #134928

    Purpose

    Fluorescently tagged GTPase.

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1B, Cerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

    Plasmid

    #126679

    Purpose

    Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

    Depositor

    Hee Won Yang

    Article

    Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

    Insert

    Rb (a.a 886-928)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pShuttle-CMV-iRFP

    Plasmid

    #31856

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

    Plasmid

    #193049

    Purpose

    Encodes G-418 resistance

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    prsp-27::NeoR::unc-54 3’ UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    prsp-27

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191537

    Purpose

    Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-ddMisCas13b-SNAPf-NLS-3xFlag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge