We narrowed to 146,921 results for: ins;

1. Lamp1-mCherry-RUSH

   Plasmid

   #202799

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

   Depositor

   Juan Bonifacino

   Article

   Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

   Insert

   Lamp1 (Lamp1 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. VRK1_HUMAN_D0

   Plasmid

   #79684

   Purpose

   This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VRK1 (VRK1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pBABE-Flag-Cdk9-D167N-IRES-eGFP

   Plasmid

   #28098

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains D167N mutation in Cdk9

   Promoter

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pBABE-Flag-Cdk9-T186A-IRES-eGFP

   Plasmid

   #28097

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains T186A mutation in Cdk9

   Promoter

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCdOpt-BMX

   Plasmid

   #203929

   Purpose

   This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

   Depositor

   Scott Briggs

   Article

   Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

   Insert

   BleMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   BleMX was codon optimized for Candida albicans or…

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

   Plasmid

   #35510

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV306DIhsp+LuxG13

   Plasmid

   #49999

   Purpose

   Integrase removed to increase stability of the plasmid in mycobacteria

   Depositor

   Brian Robertson

   Article

   Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   G13 from M. marinum G13 promoter driving luxC

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FLuc

   Plasmid

   #83281

   Purpose

   recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   Firefly Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Myc CDC4 WT*

   Plasmid

   #16652

   Depositor

   Bert Vogelstein

   Article

   Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

   Insert

   CDC4 (FBXW7 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   *Note that this insert is only WT relative to the…

   Promoter

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. BLADE

    Plasmid

    #134912

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system

    Depositor

    Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNCST-avGFP

    Plasmid

    #129508

    Purpose

    Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    avGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CKIIa-stGtACR1-FusionRed

    Plasmid

    #105679

    Purpose

    Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-MjCouRS

    Plasmid

    #229066

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    Methanococcus jannaschii tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. VRK1-dTag-puro

    Plasmid

    #199651

    Purpose

    Expresses VRK1 C-term. Tagged with dTAG

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    dTAG

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX Zap70(NC)-SH2

    Plasmid

    #46535

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Zap70(NC) (ZAP70 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-268

    Promoter

    Tac

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAL-SH3(2)-5R

    Plasmid

    #112089

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-5R (NCK1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only repeats of the second SH3…

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid

    #154141

    Purpose

    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    R887E

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-DNM2-R465W

    Plasmid

    #155262

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 465 to Tryptophan

    Promoter

    Available Since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35516

    Purpose

    AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    Arch3.0-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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