We narrowed to 13,163 results for: CAR

1. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRH2502

   Plasmid

   #84379

   Purpose

   Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   dcas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Aspartate at position 10 changed to Alanine, Hist…

   Promoter

   uv15tetO

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXs_FLAG-NMNAT1

   Plasmid

   #133259

   Purpose

   Express FLAG-tagged NMNAT1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NMNAT1 (NMNAT1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDM042

   Plasmid

   #216812

   Purpose

   Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mScarlet3
   

   Tags

   VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMM534

   Plasmid

   #112530

   Purpose

   pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Insert

   luxCDABE
   

   Expression

   Bacterial

   Promoter

   PL(HrtO)

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. bNS2B47NS3

   Plasmid

   #155320

   Purpose

   co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

   Plasmid

   #132403

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PLX306 iCre tetO tomato rtTA

   Plasmid

   #158050

   Purpose

   lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   tdTomato
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-Cre stuffer v3

   Plasmid

   #158030

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

    Plasmid

    #114322

    Purpose

    Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFR-miRFP670nano3

    Plasmid

    #184673

    Purpose

    EGF receptor (EGFR) labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    EGFR-miRFP670nano3
    

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-HA-metap2

    Plasmid

    #100512

    Purpose

    Expresses HA tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Syn-VARNAM2

    Plasmid

    #195520

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCLXEBR-pTF-hM3Dq

    Plasmid

    #114283

    Purpose

    TET-inducible expression of the hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3-SREBP-2-GFP

    Plasmid

    #204202

    Purpose

    Mammalian expression of SREBP-2

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-2 (SREBF2 Human)
    

    Tags

    T7x3 and eGFP

    Expression

    Mammalian

    Mutation

    multiple silent mutations

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-p62-Silent_Mutation_WT

    Plasmid

    #187986

    Purpose

    human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation for resistance to sip62 #5

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag Nprl2 pRK5

    Plasmid

    #46333

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEHAC1-CHIP-BirA-HA

    Plasmid

    #232587

    Purpose

    Express BirA tagged to the C-terminus of CHIP.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    CHIP - BirA (CHIP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CSII-IDR2-rhesusTRIM5-FOS

    Plasmid

    #79059

    Purpose

    Express rhesus TRIM5 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesusTRIM5-FOS
    

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge