We narrowed to 11,647 results for: emb

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pOPTM p62 PB1 ZZ (1-167)

Plasmid

#210429

Purpose

Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

Depositor

Carsten Sachse

Publication

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 ZZ (1-167) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk3b

Plasmid

#208986

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Publication

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3b (kcnk3b Zebrafish)

Use

Cloning

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80-421)

Plasmid

#209005

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pOPTM p62 PB1 (1-102)

Plasmid

#210427

Purpose

Bacterial expression of MBP-PB1(1-102) with TEV cleavage site

Depositor

Carsten Sachse

Publication

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-102) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDM767

Plasmid

#207425

Purpose

Extrachromosomal expression HSPC300-GFP and PH-CRAC-mRFPmars

Depositor

Rob Kay

Publication

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
CRAC

Use

Unspecified

Tags

GFP and mRFPmars

Mutation

aa 1–126 (PH domain) only

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-HA

Plasmid

#209237

Purpose

Mammalian expression of ATP11C

Depositor

Hye-Won Shin

Publication

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Retroviral

Tags

HA

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJL1-sfGFP-AQNAT

Plasmid

#198211

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_AQNAT

Tags

6x His Tag and AQNAT mutated (non-permissible) gl…

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtU6-Y

Plasmid

#197779

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtU6 promoter for sgRNA assembly

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-OsU6-X

Plasmid

#197780

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsU6 promoter for sgRNA assembly

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-DGK1-3HA

Plasmid

#198595

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

CDS1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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EMC1-2EGFP-ter-NAT

Plasmid

#184763

Purpose

Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

EMC1 (EMC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Hyper7 in pT3TS-Dest

Plasmid

#194296

Purpose

In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

In vitro transcription of mrna

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP4

Plasmid

#178070

Purpose

Insect cell expression of GCP4

Depositor

Tarun Kapoor

Publication

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP4 (TUBGCP4 Human)

Expression

Insect

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Publication

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Expression

Worm

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-mWasabi-G

Plasmid

#164170

Purpose

Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

Depositor

Isei Tanida

Publication

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Expression

Mammalian

Promoter

tetO

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-mWasabi-G4

Plasmid

#164168

Purpose

Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Publication

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-Y336W

Plasmid

#160515

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y336W

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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