We narrowed to 12,758 results for: sequence

1. pLKO.1 neo

   Plasmid

   #13425

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

   Depositor

   Sheila Stewart

   Insert

   non-hairpin 18bp
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-GBDpol

   Plasmid

   #165501

   Purpose

   Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Pyrococcus Sp. Heat-Stable DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGR420

   Plasmid

   #213782

   Purpose

   Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank

   Depositor

   Chang Liu

   Insert

   MET15
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE7-SB

   Plasmid

   #228869

   Purpose

   PE7 connected to MLH1-SB via 2A sequence

   Depositor

   Sangsu Bae

   Insert

   PE7-2A-MLH1-SB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-NotIR (GC)

   Plasmid

   #165507

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   NotI (GC-rich recognition site)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOpen-SfiIR (cDNA)

   Plasmid

   #165528

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   SfiI (used to create cDNA libraries)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pTRIPZ (M)-HT-NLS

   Plasmid

   #82518

   Purpose

   Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

   Depositor

   Xiaojun Ren

   Insert

   Nuclear localizaition sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCMV3

   Plasmid

   #85711

   Purpose

   Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences

   Depositor

   Martin Messerle, Ulrich Koszinowski

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MIEPmouse CMV MIEP

   Available since

   April 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZa-STE2-1D4

   Plasmid

   #133436

   Purpose

   Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C59S/C252A

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPICZac-STE2-1D4

    Plasmid

    #133437

    Purpose

    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S/C252A

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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