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Plasmid#177419PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bcl2-cb5-pEGFP-C1
Plasmid#177422PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-B3GALT5
Plasmid#192719PurposeGateway entry vector encoding human B3GALT5DepositorInsertB3GALT5 (B3GALT5 Human)
UseGateway entry vectorTagsExpressionMutationM85TPromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp
Plasmid#185061PurposeEf1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445DepositorInsertU6:pegRNA:scaffold:PBS+RT template
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsMex3C_1-225_AD
Plasmid#148497PurposeMammalian Expression of HsMex3C_1-225DepositorInsertHsMex3C_1-225 (MEX3C Human)
UseTagsExpressionMammalianMutationone non silent mutation compared to variant 2 fro…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsYTHDC2_1-1068_AD
Plasmid#148491PurposeMammalian Expression of HsYTHDC2_1-1068DepositorInsertHsYTHDC2_1-1068 (YTHDC2 Human)
UseTagsExpressionMammalianMutationone non silent mutation D233G and a deletion of 2…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsYTHDC2_1069-1430_AD
Plasmid#148492PurposeMammalian Expression of HsYTHDC2_1069-1430DepositorInsertHsYTHDC2_1069-1430 (YTHDC2 Human)
UseTagsExpressionMammalianMutationone non silent mutation L1409Q annotated as natur…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2151 pHAGE-hSPC-TFEB-TagBFP-W
Plasmid#188722PurposeLentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion proteinDepositorInsertTFEB (TFEB Human)
UseLentiviralTagstagBFPExpressionMutationPromoterhSP-CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) HuTF-Fc-His tag
Plasmid#183253PurposeSoluble expression of the extracellular domains of human tissue factor (TF)-FcDepositorInsertTissue factor (F3 Human)
UseTags8xHisExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
T11-HA-hORF1
Plasmid#185053Purposeexpression of human L1 ORF1 proteinDepositorInsertL1-hORF1
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2iC6FAST1nls2ER4(#638)
Plasmid#184068Purposecyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInsertYFAST-2nls-ERT2
UseSynthetic BiologyTagsExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSTG4FER3(#190)
Plasmid#184069Purposecyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesisDepositorInsertGal4bdFF-ERT2
UseSynthetic BiologyTagsExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-puro-hCASP8
Plasmid#185378PurposeFor mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)DepositorInsertCASP8 (CASP8 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-puro-hARAF-1
Plasmid#185367PurposeFor mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAFDepositorInsertARAF (ARAF Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-PPP2R5A-1
Plasmid#179352PurposeshRNA suppressionDepositorInsertB56α
UseLentiviralTagsExpressionMammalianMutationPromoterAge1Available sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-PPP2R5C-2
Plasmid#179356PurposeshRNA suppressionDepositorInsertB56γ
UseLentiviralTagsExpressionMammalianMutationPromoterAge1Available sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-PPP2R3B-1
Plasmid#179360PurposeshRNA suppressionDepositorInsertPR48
UseLentiviralTagsExpressionMammalianMutationPromoterAge1Available sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CNN3A-c007
Plasmid#175457PurposeProtein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.DepositorInsertCNN3 (CNN3 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLA2G6_Halo_C_allele
Plasmid#178166PurposeDonor vector for endogenous tagging of human PLA2G6 at the C-terminus with halotagInsertHalotag (PLA2G6 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
KCNC3_Halo_N_allele
Plasmid#178129PurposeDonor vector for endogenous tagging of human KCNC3 at the N-terminus with halotagInsertHalotag (KCNC3 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only