We narrowed to 17,521 results for: emb

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pMaCTag-12

Plasmid

#119991

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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iRFP-PAmCherry1-PH_GRP1

Plasmid

#92168

Purpose

PI(3,4,5)P3 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PHgrp1

Tags

iRFP-PAmCherry1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Tags

6His-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMB-TPr

Plasmid

#61190

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLP3

Plasmid

#209958

Purpose

Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-moxGFP

Plasmid

#207688

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mTB3-Myc tag

Plasmid

#210205

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

Myc-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP6

Plasmid

#178072

Purpose

Insect cell expression of GCP6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP6 (TUBGCP6 Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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P-miR-145 (pTransluc-miR-145-1)

Plasmid

#21496

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

1.5kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pHD1

Plasmid

#30974

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD1

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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TagBFP2- PIP4K2CD280K

Plasmid

#202731

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2C (D280K) (PIP4K2C Human)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Zeo

Plasmid

#207740

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xHA

Plasmid

#207707

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pACEBac1-GCP4

Plasmid

#178070

Purpose

Insect cell expression of GCP4

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP4 (TUBGCP4 Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-Osh2-PH

Plasmid

#71370

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pFUS_B10

Plasmid

#31027

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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TOPO CFP

Plasmid

#68489

Purpose

TOPO construct expressing CFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cyan Fluorescent Protein

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pL2-GGASPAGG

Plasmid

#137102

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGASPAGG

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2- GL(GGGS)3ETG

Plasmid

#137106

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GL(GGGS)3ETG

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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