We narrowed to 44,705 results for: cha

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pCAG-hChR2(H134R)-EYFP

Plasmid

#114367

Purpose

expresses channelrhodopsin 2 fused with EYFP

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

hChR2(H134R)

Tags

EYFP

Expression

Mammalian

Mutation

the Histidine at amino acid position 134 was chan…

Promoter

pCAG

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTW073

Plasmid

#64754

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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hRIP1 HA GFP K45A

Plasmid

#41389

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

Kinase Domain Mutant (K45A)

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
ZJOM144

Plasmid

#133630

Purpose

Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pTPI1-SS-GFP-HDEL

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

Plasmid

#79373

Purpose

Recruits VPR to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

Plasmid

#79372

Purpose

Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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