We narrowed to 16,040 results for: GRN

1. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126753

   Purpose

   Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126756

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128347

   Purpose

   pAAV encoding control gRNA for CRISPR gRNAs listed above

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   control (negative)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128345

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrnb4 (Chrnb4 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128341

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna6 (Chrna6 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121815

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121820

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124283

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

12. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124285

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

13. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124284

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

    Plasmid

    #113696

    Purpose

    U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

    Plasmid

    #109315

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    Mttp gRNA
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

    Plasmid

    #113695

    Purpose

    U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

    Plasmid

    #87572

    Purpose

    PV4 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA

    Plasmid

    #89055

    Purpose

    PV2 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA

    Plasmid

    #89053

    Purpose

    PV1 gRNA entry vector for CRISPR decoder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

    Plasmid

    #79380

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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