We narrowed to 20,285 results for: ACE;

1. pSB1K3 containing mRFP1

   Plasmid

   #118082

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, mRFP1
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPP5951

   Plasmid

   #128719

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

   Insert

   hopR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AktAR2-NLS

   Plasmid

   #175012

   Purpose

   Nuclear-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   AktAR-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX311 hcRed1

   Plasmid

   #117733

   Purpose

   Open reading frame vector encoding hcRed1

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   hcRed1
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK570

   Plasmid

   #110615

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP C1 SADBs

   Plasmid

   #67154

   Purpose

   pEGFP containing N-terminally EGFP Tagged mouse SADBs

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   Brsk1 (Brsk1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Use

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CDA1-BE3

   Plasmid

   #100804

   Purpose

   Expresses CDA1-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   CDA1-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21b-Caspase-3(D3A: D175A D28A D9A)

   Plasmid

   #90090

   Purpose

   Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

   Depositor

   Clay Clark

   Article

   Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A

   Promoter

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

    Plasmid

    #127314

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-CanlonicSF

    Plasmid

    #188390

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    NLS-CanlonicSF
    

    Use

    Tags

    NLS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK520

    Plasmid

    #110604

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_tKiMBI-T2A-caMEK

    Plasmid

    #199578

    Purpose

    Expresses tKiMBI and caMEK in an AAV vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
    constitutively active MEK
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMx PylT_FLAG-Mx PylRS YA

    Plasmid

    #140012

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A

    Promoter

    EF1

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GW1-mito-roGFP1-mApple

    Plasmid

    #107218

    Purpose

    Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    roGFP1-mApple
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid

    #165434

    Purpose

    Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyn-Akt-STOPS

    Plasmid

    #175009

    Purpose

    Plasma membrane-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    Lyn-mCherry-Akt-STOPS
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid

    #207756

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

    Plasmid

    #199585

    Purpose

    Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
    AkaLuc
    Blasticidin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTHMT_hnRNPF_FL

    Plasmid

    #139114

    Purpose

    express FL hnRNPF with N-terminal maltose binding protein tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPF (HNRNPF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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