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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,009 results for: ADA

Showing: 6101 - 6120 of 10009 results

  1. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Expression
    Mutation
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  7. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    131312-plasmid-map-sequence-id-254654

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  9. p-mCherry-N1-iChloC_CA

    Plasmid
    #98171
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iChloC_C128A gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E83Q, E90R, E101S, C128A, T159C, D156N
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98171-plasmid-map-sequence-id-182725

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  10. pKK-mEGFP-TEV

    Plasmid
    #105777
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mEGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105777-plasmid-map-sequence-id-203366

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  12. pRSET-XS-VWF

    Plasmid
    #64847
    Purpose
    Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus
    Depositor
    Sriram Neelamegham
    Article
    Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.
    Insert
    Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
    Use
    Tags
    7X His, Cerulean, and Venus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64847-plasmid-map-sequence-id-109340

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  13. pDisplay-GRAB_NPYmut

    Plasmid
    #208678
    Purpose
    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208678-plasmid-map-sequence-id-412165

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  18. pR26-mBMP7

    Plasmid
    #127374
    Purpose
    Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Insert
    BMP7 (Bmp7 Mouse)
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127374-plasmid-map-sequence-id-247972

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  19. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid
    #185289
    Purpose
    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    iLID::eGFP::FTH1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185289-plasmid-map-sequence-id-363896

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Showing: 6101 - 6120 of 10009 results