We narrowed to 6,532 results for: nls

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pCH68 (dAsCas12a-3xKRAB piggyBac)

Plasmid

#217334

Purpose

piggyBac expression of dAsCas12a-3xKRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

dAsCas12a

Use

CRISPR

Tags

nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

Expression

Mammalian

Mutation

E993A

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYN2_1

Plasmid

#184757

Purpose

Plasmid for genome editing by CRISPR/Cas9

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

Cas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme

Use

Synthetic Biology

Tags

2xNLS

Expression

Bacterial and Yeast

Promoter

PGK1 and tRNA-Phe-HDV Ribozyme

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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MMLV-RT for Split-PE (pEH45)

Plasmid

#190105

Purpose

Expresses MMLV-RT (full-length, from PE2) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT

Tags

bpNLS

Expression

Mammalian

Mutation

RT pentamutant from PE2 (D200N, T306K, W313F, T33…

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJ23119-sgRNA

Plasmid

#113654

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter J23119 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AA011

Plasmid

#215973

Purpose

Fragmid fragment: (N' terminus) for C>T base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA010

Plasmid

#216038

Purpose

Fragmid fragment: (C' terminus) for C>T base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

UGI_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA013

Plasmid

#215975

Purpose

Fragmid fragment: (N' terminus) for A>G base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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sABE v3.22 Lenti_1

Plasmid

#209555

Purpose

pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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AA295

Plasmid

#215986

Purpose

Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; SV40NLS_v1.19

Use

Fragment

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Available Since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3_001

Plasmid

#123952

Purpose

Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3XNLS::CRY2PHR::p65

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_001

Plasmid

#123924

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TU-sgRNA - L1/RE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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iPE-D10A-Cas9

Plasmid

#239380

Purpose

For inverse prime editor using nCas9-D10A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AA018

Plasmid

#215976

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; VP64_v1.1

Use

Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA093

Plasmid

#216042

Purpose

Fragmid fragment: (C' terminus) ZIM3 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; KRAB_v3.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA092

Plasmid

#216041

Purpose

Fragmid fragment: (C' terminus) ZIM3 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; KRAB_v2.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA122

Plasmid

#215980

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; VP64_v1.1; Linker_v3.1; SV40NLS_v1.6

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA091

Plasmid

#215979

Purpose

Fragmid fragment: (N' terminus) ZNF10 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; KRAB_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA088

Plasmid

#215978

Purpose

Fragmid fragment: (N' terminus) ZIM3 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; KRAB_v2.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge