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mut GBP2 V5-Twist

Plasmid

#201377

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

Y107E

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 47kDa

Plasmid

#201370

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-3PrA

Expression

Mammalian

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Control V5-Twist

Plasmid

#201376

Purpose

Expresses Twist for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 34kDa

Plasmid

#201339

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-1PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 54kDa

Plasmid

#201349

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-4PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 47kDa

Plasmid

#201348

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-3PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 47kDa

Plasmid

#201343

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-3PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 41kDa

Plasmid

#201340

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 41kDa

Plasmid

#201345

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMin-DHS-4D-GFP

Plasmid

#159653

Purpose

Reporter plasmid for the DHS-4D Otx2 enhancer element.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

DHS-4D

Expression

Mammalian

Promoter

TATA

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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RBMS2_2xRRM_pGEX

Plasmid

#135132

Purpose

Recombinant expression of RBMS2 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS2 (RBMS2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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KHDRBS3_KH_pGEX

Plasmid

#135133

Purpose

Recombinant expression of KHDRBS3 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

KHDRBS3 (KHDRBS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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myc-Rapr-FAK

Plasmid

#25926

Depositor

Klaus Hahn

Publication

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse)

Tags

Myc

Expression

Mammalian

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-hSyn-GRAB-rACh1h

Plasmid

#234412

Purpose

Expresses the red ACh sensor GRAB rACh1h in neurons

Depositor

Yulong Li

Publication

Xie et al Nat Neurosci. 2026 Jun 16. doi: 10.1038/

Insert

GRAB-rACh1h

Use

AAV

Promoter

hSyn

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1_Bxb1-GA_LP_Dual_TC

Plasmid

#231943

Purpose

AAVS1 targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Publication

van der Vaart et al Stem Cell Res. 2026 Jun 6;95:104028. doi

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Mutation

N/A

Promoter

PGK

Available since

June 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-mCherry

Plasmid

#240538

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.

Depositor

Wensheng Wei

Publication

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F2

Plasmid

#236432

Purpose

transient overexpression of E2F2 in mammalian cells

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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