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Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCEP4-myc-PDGFRalpha-V242K
Plasmid#136436PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationV242K (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-13 ONE-GO
Plasmid#189740PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationL342M (present in YFP but no functional consequen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- SIV-MAC251 vpx
Plasmid#115810PurposeEncodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance proteinDepositorInsertSIVMAC251 Vpx
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Alpha
Plasmid#171267PurposeMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunitDepositorInsert3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- HIV2-ROD vpx
Plasmid#115816PurposeEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance proteinDepositorInsertHIV2ROD Vpx (M15390)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR
Plasmid#188377PurposeLentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCP FLAG B55 alpha
Plasmid#13804DepositorInsertPP2A B55 alpha Flag (PPP2R2A Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human alpha5 full
Plasmid#221395PurposeMammalian expression of human integrin alpha5 full lengthDepositorInsertintegrin alpha5 full length (ITGA5 Human)
UseTagsCD33 secretion peptide (MPLLLLLPLLWAGALA)ExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i1 ONE-GO
Plasmid#204333PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i1 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-tdTomato
Plasmid#107714PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-tdTomato
UseAAVTagsKA2 and tdTomatoExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein
Plasmid#181715PurposeGalactose inducible expression of Alpha-synucleinDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i2 ONE-GO
Plasmid#189736PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport RXR alpha
Plasmid#8882DepositorInsertRXR alpha (RXRA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaz-RLuc8
Plasmid#140978PurposeEncodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaz-Rluc8 (GNAZ Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)
Plasmid#221997PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: S1330I, A1341V, N1342S, E1370K, T1380IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha Wildtype
Plasmid#163365PurposeExpress HA- and Clover-tagged HIF-1alpha wildtype plasmidDepositorInsertHypoxia Inducible Factor 1 alpha (HIF1A Human)
UseTagsHA- and Clover-taggedExpressionMammalianMutationWildtypePromoterCMV PromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pcDNA3
Plasmid#18956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only