We narrowed to 4,553 results for: pcr

1. pCRISPaint-T2A-Gal80-3xP3-RFP

   Plasmid

   #127562

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal80
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-T2A-LexGAD-3xP3-RFP

   Plasmid

   #127558

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   LexGAD
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

   Plasmid

   #127564

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4DBD
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRII-Topo Vglut2 in situ probe

   Plasmid

   #45639

   Depositor

   Jeffrey Macklis

   Article

   Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

   Insert

   Vglut2 in situ probe (Slc17a6 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains nt 2477-2993 of slc17a6 (Vglut2…

   Promoter

   Available Since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCRISPaint-T2A-p65-3xP3-RFP

   Plasmid

   #127565

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-T2A-QF2-3xP3-RFP

   Plasmid

   #127559

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-T2A-Nluc-3xP3-RFP

   Plasmid

   #127563

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Nluc
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRII-Topo Plexin D1 in situ probe

   Plasmid

   #45621

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

   Insert

   Plexin D1 in situ probe (Plxnd1 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 5751-6406 of NM_026376.3

   Promoter

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCru5-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49226

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-Topo Cav1 in situ probe

    Plasmid

    #45624

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Cav1 in situ probe (Cav1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1472-2124 of AB029929.1

    Promoter

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-Blunt-U6-HES7-STOP-gRNA

    Plasmid

    #130933

    Purpose

    gRNA for tag human HES7

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7-STOP-gRNA (HES7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCru5-/CGA-mEGFP-IRES-mCherry

    Plasmid

    #49224

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRII-HSV2.3 TCR beta chain

    Plasmid

    #74077

    Purpose

    gBT-I TCR beta chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR beta cDNA clone HSV2.3 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR3-vsv-GCN4-dCEN-INCENP

    Plasmid

    #108510

    Purpose

    expression of vsv-GCN4-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    GCN4-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    replace CEN box for GCN4 dimerazation domain

    Promoter

    CMV

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRII-Topo Ptn in situ probe

    Plasmid

    #45632

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Ptn in situ probe (Ptn Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1241-1830 of NM_008973

    Promoter

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCru5-/UAA-mEGFP-IRES-mCherry

    Plasmid

    #49225

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRII-Topo S100a10 in situ probe

    Plasmid

    #45603

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    s100a10 in situ probe (S100a10 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 21-275 of BC025044

    Promoter

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCRII-Topo Foxp2 in situ probe

    Plasmid

    #45620

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Foxp2 in situ probe (Foxp2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp#1709-2142 of AF339106

    Promoter

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid

    #171813

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge