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mCrimson2-H2B-6

Plasmid

#56188

Purpose

Localization: Nucleus/Histones, Excitation: 588, Emission: 615

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mCrimson2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCrimson3-H2B-C-10

Plasmid

#56196

Purpose

Localization: Nucleus/Histones, Excitation: 588, Emission: 615

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mCrimson3

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CAG-FLEX-Chrimson-TdTomato

Plasmid

#209202

Purpose

Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

Depositor

Larry Zweifel

Publication

Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

Insert

Chrimson

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CamkII-tTA-2A-E2-Crimson-WPRE-pA

Plasmid

#183708

Purpose

AAV designed to express tTA and-E2 Crimson from a CamKIIa promoter

Depositor

Jonathan Ploski

Has service

AAV8

Insert

tTA

Use

AAV

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Publication

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Publication

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Publication

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ADEPT-pTarget-tetR

Plasmid

#238043

Purpose

Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.

Depositor

Lingchong You

Publication

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

ttrB

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [ChrimsonR-GFP]

Plasmid

#118295

Purpose

AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CAG

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-mTagBFP-ST

Plasmid

#258875

Purpose

Expression of soma-targeted ChrimsonR fused to mTagBFP

Depositor

Hillel Adesnik

Insert

ChrimsonR

Tags

mTagBFP

Expression

Mammalian

Promoter

CAG

Available since

Sept. 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-Chrimson-mRuby2-ST

Plasmid

#258876

Purpose

Expression of soma-targeted Chrimson fused to mRuby2

Depositor

Hillel Adesnik

Insert

Chrimson

Tags

mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-mTagBFP-ST

Plasmid

#258874

Purpose

Expression of soma-targeted ChrimsonR fused to mTagBFP

Depositor

Hillel Adesnik

Insert

ChrimsonR

Tags

mTagBFP

Expression

Mammalian

Promoter

CAG

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::CHRIMSON_CE

Plasmid

#232603

Purpose

Pan-neuronal expression of opsin 'Chrimson', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Publication

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Chrimson

Expression

Worm

Mutation

N/A

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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RAP2A-p2A-E2Crimson

Plasmid

#83940

Purpose

Lentiviral vector expressing RAP2A-p2A-E2Crimson

Depositor

David Sabatini

Publication

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SLC35B2-p2A-E2Crimson

Plasmid

#83941

Purpose

Lentiviral vector expressing SLC35B2-p2A-E2Crimson

Depositor

David Sabatini

Publication

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Dissostichus mawsoni cryaa

Plasmid

#46167

Purpose

Recombinant protein production

Depositor

Mason Posner

Publication

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin

Expression

Bacterial

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

Plasmid

#113857

Purpose

For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

Depositor

Richard S Myers

Publication

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

Humanized Beta

Use

Lentiviral

Tags

P2A/Crimson

Mutation

"humanized" Beta gene

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

Plasmid

#128588

Purpose

AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

EF1α (1.1 kb short version)

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Cre-responsive Cas9 expressing transposon library vector

Plasmid

#164138

Purpose

Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

Depositor

Hyongbum Kim

Publication

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, EF-1a

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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